SubjectsSubjects(version: 912)
Course, academic year 2022/2023
Histology, molecular biology and genetics: Laboratory - M320050
Title: Laboratoř histologie, molekulární biologie a genetiky
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2022
Semester: both
Points: 3
E-Credits: 3
Examination process:
Hours per week, examination: 0/5, MC [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type: Master's (post-Bachelor)
Note: you can enroll for the course in winter and in summer semester
Guarantor: Honzík Tomáš prof. MUDr. Ph.D.
Annotation -
Last update: Pátková Vlasta (12.02.2021)
Principles of molecular genetics diagnostics of patients with monogenic disorder. Students will learn methods of Sanger and massive parallel sequencing.
Aim of the course -
Last update: Pátková Vlasta (12.02.2021)

Students will be able to isolate DNA and prepare a PCR product for Sanger sequencing. They will be able to explain Sanger sequencing reaction and to prepare a library for massive parallel sequencing. Students will be able to analyze the results of sequencing.

Literature - Czech
Last update: Pátková Vlasta (12.02.2021)

HISTOLOGICKÁ TECHNIKA - M. Jirkovská, Praha, Galén 2006, 2010

Junquierovy základy histologie - A.L.Mescher, 14.vydání, 1. české, Praha, Galén, 2018

Syllabus -
Last update: Pátková Vlasta (12.02.2021)

Introduction to safety instructions

Introduction – principles of methods

Computer work – sequence retrieval, gene structure, primer localization, PCR product length

Laboratory work – DNA isolation

Laboratory work – DNA concentration measurement

Laboratory work – PCR

Laboratory work – Agarose electrophoresis – quality control of the PCR product

Laboratory theoretical work – Sequencing reaction preparation

Computer work – Sanger and massive parallel sequencing results analysis including its clinical interpretation

General histology – microscopic structure of tissues

Special histology – microscopic structure of organs

Sampling, fixation and analysis of materials for histology laboratory techniques

Laboratory practicum – microtom and cryostat techniques

Basic and special staining methods

Laboratory practicum – staining of tissue slides by hematoxylin and eosin method

Principles of histochemistry and immunohistochemistry

Laboratory practicum – detection of polysaccharides and lipids

Laboratory practicum – immunohistochemical detection

Basic microscopy, overview of microscopic methods

Laboratory practicum – microscopy of slides prepared during the practicum

Laboratory practicum – demonstration of confocal microscope and the digital microscopy laboratory

Course completion requirements - Czech
Last update: Pátková Vlasta (15.02.2021)

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