SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Chromatography and mass spectrometry: Laboratory - M323022
Title: Laboratoř chromatografie a hmotnostní spektrometrie
Guaranteed by: Department of Food Analysis and Nutrition (323)
Actual: from 2019
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:0/5 MC [hours/week]
Capacity: unknown / unlimited (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Guarantor: Dvořáková Darina Ing. Ph.D.
Džuman Zbyněk Ing. Ph.D.
Interchangeability : N323055
Examination dates   Schedule   
Annotation -
Last update: Džuman Zbyněk Ing. Ph.D. (25.01.2018)
Subject Chromatography and mass spectrometry: Laboratory is designed for students who have completed course Interpretation of chromatographic and mass-spectrometric data. The aim of the course is to practically introduce to students modern techniques for detection of compounds utilizing various instruments, data handling and interpretation, including statistical data processing (multivariate analysis). Laboratories are designed as single handle activities of students on their own projets.
Aim of the course -
Last update: Džuman Zbyněk Ing. Ph.D. (25.01.2018)

Students will be able to:

Prepare and plan experimental work in the modern laboratory, under the leadership perform the instrumental analysis, process the interpret the acquired data.

Literature -
Last update: Džuman Zbyněk Ing. Ph.D. (25.01.2018)

R: Picó Y.: Food Toxicants Analysis, Elsevier, 2007, ISBN: 978-0-444-52843-8.

R: Otleş S.: Handbook of Food Analysis Instruments, CRC, 2008, ISBN: 9781420045666.

R: de Hoffmann E., Stroobant V.: Mass Spectrometry - Principles and Applications, Wiley, 2002 ISBN: 9780471485667.

R: Kaliszan R.: Structure and Retention in Chromatography: A Chemometric Approach (Chromatography: Principles & Practice Series), CRC, 1997 ISBN: 9789057020285.

Learning resources -
Last update: Džuman Zbyněk Ing. Ph.D. (25.01.2018)

https://web.vscht.cz/poustkaj/

http://www.chem.arizona.edu/massspec/

http://www.ionsource.com/tutorial/spectut/spec1.htm

http://www.chemguide.co.uk/analysis/masspecmenu.html

http://webbook.nist.gov/chemistry/

http://www.chemspider.com/

http://www.massbank.jp/

http://metlin.scripps.edu/

Requirements to the exam -
Last update: Vlčková Martina Ing. (30.01.2018)

Within the extent of the syllabus.

Syllabus -
Last update: Džuman Zbyněk Ing. Ph.D. (25.01.2018)

1. Introduction, organization of laboratories, laboratory safety.

2-4. Project preparation (general introduction, practical introduction in the laboratory).

5. Task entry, individual preparation (literature search, procedures proposals).

6-12. Laboratory work - work on the project.

13. Project results processing.

14. Presentation of results, project defense.

Registration requirements -
Last update: Džuman Zbyněk Ing. Ph.D. (25.01.2018)

Interpretation of chromatographic and mass spectrometric data

Course completion requirements -
Last update: Džuman Zbyněk Ing. Ph.D. (25.01.2018)

Active participation in laboratories and final defense of the project including oral presentation.

Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0,5 14
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Obhajoba individuálního projektu 0,5 14
Práce na individuálním projektu 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 50
Defense of an individual project 50

 
VŠCHT Praha