SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Chemistry of Natural Compounds: Laboratory I - M342015
Title: Laboratoř Chemie přírodních látek I
Guaranteed by: Department of Chemistry of Natural Compounds (342)
Actual: from 2019
Semester: summer
Points: summer s.:7
E-Credits: summer s.:7
Examination process: summer s.:
Hours per week, examination: summer s.:0/10 MC [hours/week]
Capacity: unknown / unlimited (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Raich Ivan doc. Dr. Ing.
Examination dates   Schedule   
Annotation -
Last update: Fialová Jana (20.12.2017)
Lab training is designed for works whose result would be few tens of milligrams (ca 50 mg) of pure compound which is a sufficient amount for chemical characterization. The intention is to learn to work with small amounts and with special equipment and labware as is usual for research work isolating compounds of natural origin.
Aim of the course -
Last update: Fialová Jana (20.12.2017)

Students will be able to:

Isolate and purify small amounts of compounds from natural materials

Work with labware designed for semi-micro preparative scale

Design procedures and equipment suitable for work with small amounts of compounds

Perform spectral and other characterization from small amounts of isolated compounds

Literature -
Last update: Fialová Jana (20.12.2017)

R: Mayo D. W., Pike R. M., Butcher S. S.: Microscale Oganic Laboratory , John Wiley and Sons, Inc., 2nd ed., New York, 1989

Learning resources -
Last update: Fialová Jana (20.12.2017)

Raich I., Medonos M.: Instructions for Isolation and separation methods: Laboratory, ICT Prague, 2012

Syllabus -
Last update: Fialová Jana (20.12.2017)

1. Labor safety in laboratory, construction of apparatuses for basic operations

2. Isolation of caffeine from tea, extraction, sublimation, melting point

3. Isolation of trehalose from yeast, deproteinization, HPLC verification of product purity

4. Isolation of piperine from black pepper, extraction, decantation, TLC, crystallization

5. Isolation of botulin from birch bark, extraction, crystallization, derivatization

6. Isolation of cinnamaldehyde from cinnamon using steam distillation

7. Isolation of D-glucosamine from crab shells, decalcification, hydrolysis, crystallization

8. GLC analysis of cinnamaldehyde

9. HPLC analysis of trehalose on ion-exchange resin

10. NMR spectra measurements of prepared samples

11. IR spectra measurements of prepared samples

Registration requirements -
Last update: Fialová Jana (20.12.2017)

Organic chemistry I

Organic chemistry: Laboratory I

Course completion requirements - Czech
Last update: MORAVCOJ (27.02.2018)

Odevzdání preparátů a/nebo postupů vedoucímu práce.

Zpracování experimentální činnosti do podoby písemného odborného sdělení a odevzdání vedoucímu práce.

Coursework assessment
Form Significance
Regular attendance 30
Homework preparation 20
Report from individual projects 50

 
VŠCHT Praha