SubjectsSubjects(version: 955)
Course, academic year 2023/2024
Separation Methods in Biotechnologies - M409004
Title: Separační procesy v biotechnologiích
Guaranteed by: Department of Chemical Engineering (409)
Faculty: Faculty of Chemical Engineering
Actual: from 2020
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: 35 / 35 (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Přibyl Michal prof. Ing. Ph.D.
Hasal Pavel prof. Ing. CSc.
Interchangeability : AM409004, N409028, N409028A
Is interchangeable with: AM409004
This subject contains the following additional online materials
Annotation -
This course deals with traditional and modern methods of separation and purification of biotechnological products. Characteristics of biotechnology products and typical sequences of separation/purification steps for particular products will be given. Theoretical background of unit separation processes will be shown as well as typical laboratory and industrial equipment. In seminars, practical problems dealing with production of biotechnology species will be solved with the use of PCs.
Last update: Hladíková Jana (15.01.2018)
Aim of the course -

Student will be able to:

  • determine a sequence of separation steps which is necessary to obtain biotechnological products such as saccharides, proteins, nucleic acids or small metabolites.
  • construct simple mathematical models of typical separation processes.
  • solve practical tasks related to biotechnology product separation.

Last update: Hladíková Jana (15.01.2018)
Literature -

R Harrison R.G., Todd P., Rudge S.R., Petrides D.P., Bioseparations science and engineering, 2nd edition, Oxford University Press, 2015, 978-0-19-539181-7.

A Villadsen J., Lee S.Y., Nielsen J., Stephanopoulos G. (eds.), Fundamental Bioengineering, Wiley-Blackwell, 2016, 978-3527336746.

A Hu W-S., Engineering Principles in Biotechnology, Wiley, 2017, 978-1119159025.

Last update: Přibyl Michal (12.11.2018)
Learning resources -


Last update: Hladíková Jana (15.01.2018)
Syllabus -

1. Separation and purification operations in biotechnology - typical sequences. Biotechnological products.

2. Cell composition, cell structure, cell types. Cell disintegration, chemical and mechanical methods.

3. Flocculation, flocculants, electrostatic interaction, electric double layer, Debye length, effects of ionic strength, Schulze-Hardy rule.

4. Centrifugation, forces acting on settling particles, settling time and rate, devices for centrifugation, bowl centrifuge, disk centrifuge.

5. Membrane separation processes, membrane modules, mass balances, membranes, concentration polarization, intensity of permeate flux, polarization module, mass transfer coefficient, Darcy law, membrane area.

6. Supercritical extraction, p-T phase diagram, critical parameters, physical and chemical properties of supercritical fluids.

7. Devices for supercritical extraction, fractionation, mathematical models of supercritical extraction, free diffusion model.

8. Adsorption and chromatography, adsorption isotherms, adsorption in stirred vessels. Sorbent types, methods of chromatography, pressure drop in chromatography, HETP, retention time, resolution, elution in a gradient.

9. Adsorption in packed-bed systems, models of adsorption with and without axial dispersion, effective velocity of movement of species.

10. Parameterization of equilibrium model of adsorption without the axial dispersion, linear equilibrium, desorption with nonlinear equilibrium, shock wave.

11. Protein precipitation, effects of electric charge on protein solubility, effects of ionic strength and solvent composition, precipitation stages.

12. Particle size distribution of precipitate in CSTR, distribution function, growth rate, methods of precipitation, devices for precipitation.

13. Crystallization, nucleation, crystal growth, mass transfer at crystals, crystallization kinetics in batch arrangement, moments of size distribution function.

14. Lyophilization, principles, equipment, use, driving force, shrinking core model.

Last update: Hladíková Jana (15.01.2018)
Registration requirements -

Unit operations I

Mathematics I

Physics I

Last update: Hladíková Jana (15.01.2018)
Course completion requirements -

To obtain an assessment, the examination test must be completed at a minimum of 50% of the score. To complete the oral exam, the student must successfully answer two questions from the list of questions. The resulting mark is calculated as an arithmetic mean of the results of the examination test and the oral part of the exam.

Last update: Přibyl Michal (22.02.2018)
Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1.5 42
Příprava na zkoušku a její absolvování 1 28
Účast na seminářích 0.5 14
4 / 4 112 / 112