SubjectsSubjects(version: 845)
Course, academic year 2018/2019
  
Bioinformatics: Specialisation Laboratory II - N320097
Title in English: Laboratoř oboru Bioinformatika II
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2016
Semester: winter
Points: winter s.:1
E-Credits: winter s.:1
Examination process: winter s.:
Hours per week, examination: winter s.:0/2 MC [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Guarantor: Uhlík Ondřej doc. Ing. Ph.D.
This subject contains the following additional online materials
Annotation -
Last update: TAJ320 (11.12.2013)
The course provides training in the techniques used in routine molecular biology methods producing data, which are often subject to bioinformatics analysis. It aims at methods of isolation and analysis of DNA sequences and electrophoretic separation of target proteins. The course objectives further include: good laboratory practice, the rules of organization of an experiment and appropriate interpretation of data.
Aim of the course -
Last update: TAJ320 (11.12.2013)

Students will be able to:

Independently conduct and properly record particular experiment. Isolate specific DNA molecules from cells and conduct their analysis through restriction digestion and nucleotide sequencing.

Conduct and interpret basic of electrophoretic DNA and protein analyses.

Understand the importance specific working procedures in molecular biology laboratory and will be knowledgeable in options allowing optimizing selected parameters of the experiments introduced during the course.

Literature -
Last update: Kotrba Pavel prof. Ing. Ph.D. (09.07.2013)

R: Ruml T., Rumlová M., Pačes V., Genové inženýrství, VŠCHT Praha, 2002, 9788070804995

Learning resources -
Last update: Kotrba Pavel prof. Ing. Ph.D. (09.07.2013)

manufacturers´s protocols and instructions and tips at http://www.protocol-online.org/

Syllabus -
Last update: Kotrba Pavel prof. Ing. Ph.D. (02.07.2013)

1. Operational safety in laboratory, good laboratory practice and recording an experiment

2. Introduction to course organization and the proper use of specific labware and chemicals

3. Small-scale izolation of plasmid DNA

4. Restriction digestion of plasmid DNA

5. Electrophoretic analysis of the fragments of digested plasmid

6. Polymerase chain reaction (PCR)

7. Electrophoretic analysis to verify a PCR amplicon correct

8. DNA sequencing reaction

9. Analysis of sequencing products and reading the DNA sequence

10. Preparation of an extract from cells producing a recombinant protein

11. Electrophoretic separation of cell-free extract and of isolated recombinant protein

12. Recording and interpretation of the electrophoreogram of separated proteins

13. Discussion about optional experimental settings and their impact on results

14. Completion of final report

Registration requirements -
Last update: Kotrba Pavel prof. Ing. Ph.D. (09.07.2013)

none

 
VŠCHT Praha