The course covers the potential of application of isotopically (using stable as well as radioactive isotope) labeled compounds in chemistry, biology and medicine with special aspects of this discipline (synthesis in ng to mg scale, nomenclature, radiolysis, isotopic effect, hygiene of ionizing radiation)
Last update: TAJ342 (20.11.2012)
Předmět seznamuje s potenciálem použití isotopicky ( s použitím jak stabilního tak radioaktivního isotopu) značených sloučenin (ZS) v chemii, biologii a medicíně a se speciálními aspekty tohoto oboru (syntéza v měřítku ng až mg, nomenklatura, radiolýza, isotopový efekt a hygiena ionizujícího záření).
Aim of the course -
Last update: TAJ342 (30.09.2013)
Students will be able to:
Give the most important applications of labeled compounds in natural sciences research, in drug development, and in practical medicine
Name syntheses of these compounds (with emphasis on both relations and differences from usual synthetic chemistry of organic compounds)
Formulate measures due to radiation hygiene
Last update: TAJ342 (20.11.2012)
Studenti budou umět:
Zásadní aplikace ZS ve výzkumu v přírodních vědách, ve vývoji léčiv a v praktické medicíně
Popsat syntézy těchto sloučenin (s důrazem na souvislosti a na druhé straně na odlišnosti od obvyklé syntetické chemie organických sloučenin)
Zformulovat opatření v důsledku hygieny záření, které je provází
Literature -
Last update: TAJ342 (30.09.2013)
R: Navrátil O., Hála J., Kopunec R., Macášek F., Mikulaj V., Lešetický L. Nuclear chemistry, Academia, Praha 1992.
A: Evans E. A., Oldham K. G.: Radiochemicals in Biomedicinal Research , John Wiley and Sons
A: Evans E. A., Tritium and its compounds, Butterworths, 2. ed.
A: Murray A., Williams D.L.: Organ. Syntheses with Isotopes
A: Donald G.: Syntheses with Stable Isotopes
A: Thomas A. F.: Deuterium Labeling in Organic Chemistry
Last update: TAJ342 (26.09.2013)
Z: Navrátil O., Hála J., Kopunec R., Macášek F., Mikulaj V., Lešetický L. Nuclear chemistry, Academia, Praha 1992.
Z: Kupka K., Kubinyi J., Šámal M. a kol.: Nukleární medicína, p3k, Praha 2007.
Z: Chalabala M. a kol : Technologie léků /Galén 1997/
D: Evans E. A., Oldham K. G.: Radiochemicals in Biomedicinal Research , John Wiley and Sons
D: Evans E. A., Tritium and its compounds, Butterworths, 2. ed.
D: Murray A., Williams D.L.: Organ. Syntheses with Isotopes
D: Donald G.: Syntheses with Stable Isotopes
D: Thomas A. F.: Deuterium Labeling in Organic Chemistry
1. Definitions of concepts and quantities in the field of labeled compounds (LC), historical overview, importance of LC, intersections with other disciplines, literature
2. Types of radioactive transformations, kinetics of radioactive transformations, interaction of ionizing radiation (IoR) with matter, other physical principles in LC
3. Dosimetry of IoR (detectors: ionizing, scintillating, integral dosimetry methods)
4. Principles of IoR hygiene. Definition of quantities, basic knowledge of IoR effects on molecule, cell, and organism level, hygiene findings, IoR effects
5. Protection against IoR, LNT basis, ALARA access, lesson from serious nuclear disasters
6. Radio iodine, designation for immune chemical determinations (RIA, IRMA, REA, RRA, FPIA, …)
7. Specialties of LC chemistry (isotopic effect, nomenclature of LC, LC stability, specialties resulting from micro scale synthesis in mg to ng)
8. Overview of application of LC, equivalency of chemical bonds, tautomery, reaction mechanisms, biochemical pathways, internal enzyme transfer, metabolic turns, receptor study, drug development)
9. Hydrogen isotopes labeled compounds. Properties of [3H]-compounds, their synthesis