SubjectsSubjects(version: 853)
Course, academic year 2019/2020
Techniques of Measurement and Interpretation of Luminiscence Spectra - N402011
Title: Techniky měření a interpretace luminiscenčních spekter
Guaranteed by: Department of Analytical Chemistry (402)
Actual: from 2019
Semester: winter
Points: winter s.:2
E-Credits: winter s.:2
Examination process: winter s.:
Hours per week, examination: winter s.:1/0 Ex [hours/week]
Capacity: 14 / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type:  
Guarantor: Fähnrich Jan Mgr. CSc.
Annotation -
Last update: Fähnrich Jan Mgr. CSc. (27.08.2013)
The course focuses on the explanation of processes taking part in photoluminescence and affecting its analytical applications. Overview of basic applications of photoluminescence in analytical, industrial and research practice is given.
Aim of the course -
Last update: Fähnrich Jan Mgr. CSc. (27.08.2013)

Students will be able to:

Select appropriate photoluminescence method in cases where it is suitable for the solution of a given task

Use this method in correct manner acknowledging factors which may deteriorate the reliability of results

Literature -
Last update: VED402 (01.10.2013)

R: Lakowicz J.R., Principles of Fluorescence Spectroscopy, Springer, 2006, ISBN 13: 978-0387-31278-1

Powered by TCPDF (

Learning resources -
Last update: Fähnrich Jan Mgr. CSc. (27.08.2013)

Encyclopedia of Analytical Chemistry

Copyright © 1999-2013 by John Wiley and Sons, Inc.

Electronic Absorption and Luminescence

Syllabus -
Last update: Fähnrich Jan Mgr. CSc. (27.08.2013)

1. Basic concept: energetic states of molecule, Jablonski diagram

2. Franck-Condon principle, transition moment, quantum yield and quantum efficiency

3. Instrumental equipment: sources of excitation radiation, monochromators, cuvettes

4. Detection of luminescence radiation

5. Measured dependencies: excitation-emission matrix, luminescence quenching, Stern-Volmer equation

6. Measurement of quantum yield using absolute and relative methods

7. Analytical applications of luminescence: qualitative analysis, the effect of substance structure

8. Quantitative analysis

9. Luminescence in organic and inorganic analysis

10. Detection in chromatography, derivatization methods

11. Special techniques: time resolved luminescence, correlation spectrometry

12. Methods based on energy transfer, Shpolski effect

13. Low temperature spectrometry, polarization of luminescence, room temperature phosphorescence

14. Chemiluminescence, kinetic measurements, photoluminescence sensors

Registration requirements -
Last update: Fähnrich Jan Mgr. CSc. (27.08.2013)

Analytical chemistry


Teaching methods
Activity Credits Hours
Účast na přednáškách 0,5 14
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,5 14
Příprava na zkoušku a její absolvování 1 28
2 / 2 56 / 56
Coursework assessment
Form Significance
Oral examination 100