SubjectsSubjects(version: 853)
Course, academic year 2019/2020
  
Techniques of Measurement and Interpretation of Mass Spectra - N402029
Title: Techniky měření a interpretace hmotnostních spekter
Guaranteed by: Department of Analytical Chemistry (402)
Actual: from 2019
Semester: winter
Points: winter s.:2
E-Credits: winter s.:2
Examination process: winter s.:
Hours per week, examination: winter s.:1/0 Ex [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
Is provided by: M402010
For type:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Sýkora David doc. Dr. RNDr.
Zachař Pavel RNDr. CSc.
Is interchangeable with: M402010
Annotation
Last update: Sýkora David doc. Dr. RNDr. (19.09.2013)
Principles of mass spectrometry will be provided including description of hardware and software. Basic principles of ion fragmentation will be explained and interpretation of MS spectra generated by electron ionization will be covered in more detail. Current ion sources, analyzators, and detectors will be discussed. Hyphenated techniques, specifically, GC-MS and LC-MS will be covered.
Aim of the course -
Last update: Sýkora David doc. Dr. RNDr. (19.09.2013)

Students will know:

Basic principles of mass spectrometry including knowledge of hardware and software, knowledge of hyphenated techniques namely GC-MS, LC-MS, basic knowledge of MS spectra interpretation (generated by electron ionization).

Literature
Last update: VED402 (01.10.2013)

R: H.C.Hill: Introduction to Mass Spectrometry, Heyden & Son Ltd, London 1972

A: Fred W. McLafferty, František Tureček: Interpretation of Mass Spectra, University Science Books, Sausalito, California 1993, ISBN 0-935702-25-3, Fourth Edition

Learning resources -
Last update: Sýkora David doc. Dr. RNDr. (19.09.2013)

Presentations in electronic format available for students on the intranet.

Teaching methods -
Last update: Sýkora David doc. Dr. RNDr. (19.09.2013)

Presentations and excursion within the department.

Requirements to the exam -
Last update: Sýkora David doc. Dr. RNDr. (19.09.2013)

Written test.

Syllabus
Last update: Sýkora David doc. Dr. RNDr. (19.09.2013)

1. Principles of mass spectrometry, history of mass spectrometry.

2. Ionization techniques, ion separation and their detection.

3. Sector MS instruments and high resolution.

4. Quadrupole, time-of-flight, and FT MS instruments.

5. Gass chromatography-mass spectrometry (GC-MS), principles.

6. Interpretation of EI spectra of organic molecules, molecular ion, fragments.

7. Fragmentation mechanism, isotopic clusters.

8. EI spectra of selected compounds - hydrocarbons, alcohols, esters, acids, and ethers.

9. EI spectra of selected compounds - compounds containing N, S, B, and metals.

10. Interpretation of real MS spectra, MS libraries, TIC a SIM chromatograms.

11. Liquid chromatography-mass spectrometry, ionization under atmospheric pressure.

Entry requirements -
Last update: Sýkora David doc. Dr. RNDr. (19.09.2013)

Basic knowledge of analytical chemistry (ACH1 and ACH2) is necessary.

Registration requirements -
Last update: Sýkora David doc. Dr. RNDr. (19.09.2013)

Basic knowledge of analytical chemistry (ACH1 and ACH2) is necessary.

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 1 28
2 / 2 56 / 56
Coursework assessment
Form Significance
Regular attendance 10
Examination test 90

 
VŠCHT Praha