SubjectsSubjects(version: 954)
Course, academic year 2023/2024
Imunochemistry - P320006
Title: Imunochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2023
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 3/0, other [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course is intended for doctoral students only
can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Fukal Ladislav prof. Ing. CSc.
Holubová Barbora doc. Ing. Ph.D.
Is interchangeable with: AP320006
Annotation -
The course focuses on antibody-antigen interaction aspects especially in terms of in vitro characterization, resulting in a wide variety of immunoassays. After understanding the functions of the immune system and the characteristics of the structure and properties of antigens and antibodies, including their interaction, methods of targeted antibody preparation for analyzes and the characteristics of immunochemical techniques will be described. In individual projects the students will study a selected type of immunochemical technique, principles and possibilities of use. The choice of topics will either be in line with the topic of the doctoral project, or with regard to the current state of research in this area. The results will be presented in the form of short presentation during the course.
Last update: Fukal Ladislav (05.11.2018)
Aim of the course -

Students will be able to:

Understand the function of the immune system, especially with regard to the possibility of influencing the production of antibodies. Parameters of antigen-antibody interaction, the details of the various immunoassay principles.

Last update: Fukal Ladislav (09.07.2018)
Literature -

Fukal L., Holubová B.: Imunochemie a imunoanalýza. Praha 2007, ISBN 978-80-239-8903-8 (z)

Daussant J., Desvaux F.X., Introduction to Immunochemical Techniques for Medical Diagnosis, Food Quality Control and Environmental Testing, Praha, 2007, ISBN 978-80-7080-641-8 (z)

Wild D.: The Immunoassay Handbook – Theory and applications of ligand binding, ELISA and related techniques. Elsevier Ltd. 2013, Oxford, UK. ISBN: 978-0-08-097037-0 (d)

Murphy K.: Janewayś ImmunoBiology. GarlandScience New York 2011, ISBN 978-0-8153-4243-4 (d)

Last update: Holubová Barbora (15.09.2023)
Syllabus -

Lectures will be focused on the basic functions of individual components of immune system; adaptive part of the immune system (interaction of cell components leading to the final production of specific antibodies); characteristics related to the structure and properties of antigens and antibodies; methods of antibody preparation; parameters antigen-antibody interaction in-vitro; haptens and immunoassay; chemical modification of molecules of antigens and antibodies for immunoassays needs; details about principles and procedures of basic immunochemical methods (precipitation vs. non-precipitation methods, methods with a mark vs. without a mark); Enzyme Immunoassay and differences in variations of its arrangement; non-precipitation immunoassay with other marker types (than enzyme); amplification of the label signal per unit of antibody-antigen interaction; principles of multidetection antibody chips, immunosensors including microfluidic arrangements and surface plasma resonance; immunoblotting, immunohistochemical applications (including fluorescence multiplex), fluorescence flow cytometry. Immuno-affinity chromatography, user-friendly immunochemical assay formats.

Part of the course will be devoted to elaboration and presentation of individual professional projects of students.

Last update: Fukal Ladislav (05.11.2018)
Course completion requirements -

Developing and defending a project.

To pass an exam.

Last update: Fukal Ladislav (09.07.2018)