SubjectsSubjects(version: 850)
Course, academic year 2019/2020
  
Advanced imaging techniques - P320007
Title in English: Pokročilé zobrazovací techniky
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2019
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 3/0 other [hours/week]
Capacity: winter:unlimited / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: doctoral
Note: you can enroll for the course in winter and in summer semester
Guarantor: Lipov Jan Ing. Ph.D.
Zelenka Jaroslav Ing. Ph.D.
Is interchangeable with: AP320007
Annotation -
Last update: Lipov Jan Ing. Ph.D. (02.05.2018)
Microscopy techniques provide an efficient and unique approach to the study of fixed tissues and living cells with high specificity and sensitivity. Workshop aims to introduce the students to modern microscopy techniques for biology object imaging. Part of the workshop will be the introduction to automated wide-field, confocal and superresolution fluorescent microscopy. First, a brief introduction to the physical mechanisms underlying these imaging techniques will be given. Then we introduce the theoretical and/or practical basis of particular advanced imaging techniques. The basics of image analysis will be covered as well.
Aim of the course -
Last update: Lipov Jan Ing. Ph.D. (02.05.2018)

Student will be introduced to modern facts and methodology in advanced microscopy of biological objects, she/he will obtain an overview of possible techniques, which allows her/him more qualified decisions in scientific research activities.

Literature -
Last update: Lipov Jan Ing. Ph.D. (19.10.2018)

Scientific papers and online books from the portfolio of Nature Research, AAAS and others.

Reviews in Fluorescence, Geddes, Chris D., Lakowicz, Joseph R., Springer, ISSN: 1573-8086

Fluorescence Imaging and Biological Quantification, Raquel Seruca, Jasjit S. Suri, J. Miquel Sanches, CRC Press, ISBN: 9781498737043

Fluorescence Microscopy: From Principles to Biological Applications, 2nd Edition,Ulrich Kubitscheck, Wiley-Blackwell, ISBN: 978-3-527-33837-5

Advanced Fluorescence Microscopy Methods and Protocols, Verveer, Peter J., Springer, ISBN: 978-1-4939-2080-8

Super-resolution microscopy collection, Nature

web pages of microscopic companies (Zeiss, Leica, Olympus, Nikon), for example

http://zeiss-campus.magnet.fsu.edu/tutorials/

https://www.microscopyu.com/tutorials

Teaching methods -
Last update: Lipov Jan Ing. Ph.D. (13.09.2018)

Lectures, professional debate, self-study, excursion.

Syllabus -
Last update: Lipov Jan Ing. Ph.D. (02.05.2018)

Fluorescence microscopy as a specific part of optical microscopy, quantification of fluorescence, fluorescent tags.

Confocal, multiphoton and correlation microscopy.

The application of fluorescence microscopy - FRAP (Fluorescence Recovery After Photobleaching), FRET (Förster Resonance Energy Transfer)

The application of fluorescence microscopy - TIRF (Total Internal Reflection Fluorescence), SIM (Structured Illumination Microscopy)

The application of fluorescence microscopy - PALM (PhotoActivated Localization Microscopy), STORM (Stochastic Optical Reconstruction Microscopy)

The application of fluorescence microscopy - STED (Stimulated Emission Depletion microscopy), FLIM (Fluorescence-Lifetime Imaging Microscopy).

Image analysis – parameter ajdustment before acquisition, software for image processing and analysis – deconvolution, 3D visualization, colocalization, tracking, correction of chromatic abberations.

Entry requirements -
Last update: Lipov Jan Ing. Ph.D. (02.05.2018)

Basic knowledge in the field of optics and microscopic techniques.

Course completion requirements -
Last update: Lipov Jan Ing. Ph.D. (02.05.2018)

Active presence of the student on the lectures.

More then 50% from the final test.

Coursework assessment
Form Significance
Regular attendance 40
Defense of an individual project 10
Examination test 50

 
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