SubjectsSubjects(version: 955)
Course, academic year 2023/2024
Biochemical Methods - P320009
Title: Biochemické metody
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2023
Semester: summer
Points: summer s.:0
E-Credits: summer s.:0
Examination process: summer s.:
Hours per week, examination: summer s.:3/0, other [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course is intended for doctoral students only
can be fulfilled in the future
Guarantor: Lipovová Petra doc. Ing. Ph.D.
Káš Jan prof. Ing. DrSc.
Novotná Zuzana doc. Dr. Ing.
Is interchangeable with: AP320009
Annotation -
The aim of this course is to deepen the knowledge of the principles of particular biochemical techniques, whether classical or new. Part of this course will be devoted to the extension of knowledge gained in previous studies. In the second part of the course the students will present the latest techniques published in a renowned magazines. In general, the course is designed so that the graduates acquire both the key theoretical knowledge in the field and an overview of the current methodologies used in the biochemical and molecular-biological research.
Last update: Lipovová Petra (06.11.2018)
Aim of the course -

During the previous master's study students met with theoretical foundations of biochemical methods. Within this subject, their knowledge about the latest approaches and modern methodologies used in laboratory practice will be extended. Students will acquire theoretical and directly practical knowledge and skills in modern biochemical methods. It will also benefit from the enhancement of presentation skills, in which students will prepare a brief briefing on the new techniques they use in the laboratories as part of their dissertation work.

The first block of lectures will focus on classical methods - work with biological material (storage, homogenization, extraction, sample preparation for mass spectrometry), electrophoretic methods, chromatographic methods, membrane processes, centrifugation, PCR methods, methods of protein interaction analysis - depending on the students' knowledge (lectures and discussions).

The second block will be devoted to the recent techniques, students will present a short presentation of a selected method published in a renowned magazines to other participants of the course.

Last update: Lipovová Petra (04.05.2022)
Literature -

Boyer, Rodney F: Biochemistry laboratory: modern theory and techniques, Pearson, New Jersey, 2012 ISBN-13:978-0-13-604302-7

Janson JC: Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition, John Wiley & Sons, Inc., New Jersey 2011 ISBN:9780471746614

Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New Jersey, 2016, ISBN 978-1-118-30254-5

Last update: Lipovová Petra (09.07.2018)
Syllabus -

1. Source of proteins, dezintegration, membrane process

2. Precipitation, extraction, and centrifugation

3. Basic classification and instrumentation, materials for electrophoretic and chromatographic methods

4. Gel permeation chromatography, ion exchange chromatography, hydrophobic chromatography and reverse phase chromatography.

5. Affinity chromatography

6. Recombinant proteins, production and troubleshooting

7. Thin layer chromatography, monitoring of chromatography and determination of protein concentration

8. Introduction to electrophoretic methods

9. Types of electrophoretic methods

10. General principles and division of immunochemical methods

11. Immunoaffinity separation of molecules

12. Presentation of a selected method published in a scientific journal

13. Presentation of a selected method published in a scientific journal

14. Presentation of a selected method published in a scientific journal

Last update: Lipovová Petra (04.05.2022)
Entry requirements -


Last update: Lipovová Petra (09.07.2018)
Registration requirements -


Last update: Lipovová Petra (09.07.2018)
Course completion requirements -

Presentation of a review paper form the field of biochemical methods.

Oral examThe subject is finished by an oral exam.

Last update: Lipovová Petra (09.07.2018)