|
|
|
||
|
This course provides an overview of the molecular mechanisms by which pathogenic bacteria interact with their hosts. It introduces fundamental concepts in infectious disease biology, and focuses on the evolution of bacterial pathogens, as well as the strategies they use to overcome colonization barriers, and evade host immune defenses. Key principles are illustrated using examples of important human and veterinary pathogens, including foodborne bacteria. The course also covers current approaches to treatment and prevention, such as antimicrobial therapies and vaccines, as well as state-of-the-art research methods used to investigate bacterial pathogenesis.
Last update: Kamanová Jana (29.01.2026)
|
|
||
|
Z: Brenda A. Wilson, Abigail A. Salyers, Dixie D. Whitt, Malcolm E. Winkler: Bacterial Pathogenesis: A Molecular Approach, 3rd edition 2010 ASM Press, ISBN-13: 978-0632037759 Z:Kenneth Murphy, Casey Weaver: Janeway's Immunobiology, Ninth Edition (Immunobiology: The Immune System (Janeway), 2017, Garland Science, ISBN-13: 978-0815345053 D:Goering R., Dockrell H., Roitt I., et al.: Mims' Medical Microbiology, 2012, ELSEVIER, ISBN 9780808924401, 9780702056635. Last update: Kamanová Jana (29.01.2026)
|
|
||
|
Předmět je zakončen písemnou zkouškou a prezentací projektu. Last update: Kamanová Jana (29.01.2026)
|
|
||
|
1. Introduction pathogens and infectious diseases; definition of virulence; Koch's postulates and molecular Koch’s postulates; virulence factors – categories and principles of action; types of pathogens; types of hosts
2. Evolution of bacterial pathogens evolutionary pressures; horizontal genetic transfer; evolution of virulence factors
3. Ecology of bacterial pathogens within the host organism regulation of gene expression of bacterial pathogens; detection of host clues; quorum sensing; metabolites and their roles in virulence and host interaction interaction with the local microflora; bacteriocins and T6SS; acquisition of nutrients and essential metals
4. Routes of entry, modes of transmission and innate (non-specific) immunity routes of entry and modes of transmission; barrier immune processes complement system; pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs); phagocyte response
5. Pathogenicity and virulence from the perspective of the pathogen steps in successful infection and molecular mechanisms enabling bacterial pathogens to overcome innate immunity; secretion systems and cell biology of bacterial effector proteins and toxins
6. Methods of treatment antibiotics and methods of increasing sensitivity to antibiotics; phage therapy; new therapeutic targets
7. Control and prevention adaptive (specific) immunity; vaccines as tool for modulation of host response and protection from infection, mechanisms of escape; antigenic and phase variation; escape mutations
8. Research systems experimental systems for host-pathogen interaction studies; state-of-art methodology Last update: Kamanová Jana (29.01.2026)
|
|
||
|
Shared lecture slides Last update: Kamanová Jana (29.01.2026)
|
|
||
|
After completing this course, students will be able to: 1. characterize important human and veterinary pathogens, including foodborne bacteria 2. interpret experimental data and methodologies used in host–pathogen interaction studies 3. apply acquired knowledge to develop detection methods for infectious bacterial agents in food, and for the clinical diagnosis of infectious diseases 4. design and evaluate prevention strategies Last update: Kamanová Jana (29.01.2026)
|
|
||
|
Basic knowledge of biochemistry, microbiology, and molecular biology at the level taught in the bachelor’s degree program at UCT Prague. Last update: Kamanová Jana (29.01.2026)
|
| Teaching methods | ||||
| Activity | Credits | Hours | ||
| Obhajoba individuálního projektu | 0.7 | 20 | ||
| Účast na přednáškách | 1 | 28 | ||
| Práce na individuálním projektu | 0.6 | 16 | ||
| Příprava na zkoušku a její absolvování | 0.7 | 20 | ||
| 3 / 3 | 84 / 84 | |||