SubjectsSubjects(version: 948)
Course, academic year 2019/2020
  
Isolation and Characterisation of Biomacromolecules - AB320010
Title: Isolation and Characterisation of Biomacromolecules
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Teaching methods: full-time
Level:  
For type: Bachelor's
Additional information: http://biomikro.vscht.cz/vyuka/?Predmet=ib
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Lipovová Petra doc. Ing. Ph.D.
Holubová Barbora doc. Ing. Ph.D.
Interchangeability : B320010, N320018, S320018
Is interchangeable with: B320010
Examination dates   Schedule   
Annotation -
Last update: Kubová Petra Ing. (06.05.2019)
The course focuses on understanding the principles of purification techniques for the isolation of biomolecules. The aim is to teach students how to design a proper procedure for the purification of biomolecules target and characterize it. This knowledge can be used in many fields.
Aim of the course -
Last update: Kubová Petra Ing. (06.05.2019)

Students will be able to:

Understand the principles of membrane, chromatographic, electrophoretic techniques, and immunoaffinity methods.

Propose a procedure to optimize protein purification.

Literature -
Last update: Kubová Petra Ing. (06.05.2019)

R: Janson J.C.: Protein Purification, Wiley, New York, 1998, ISBN 0471186260

R: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New York, 2004, ISBN 0471544477

D: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New Jersey, 2016, ISBN 978-1-118-30254-5

D: Manz A., Dittrich P.S., Pamme N., Iossifidis D.: Bioanalytical Chemistry 2nd Edition, London, 2015, ISBN 978-1-78326-671-5

Learning resources -
Last update: Kubová Petra Ing. (06.05.2019)

e-learning.vscht.cz

Syllabus -
Last update: Kubová Petra Ing. (06.05.2019)

1. Introduction - source of proteins, dezintegration, membrane process

2. Precipitation, extraction, and centrifugation

3. Materials for chromatographic and eletrophoretic methods

4. The basic division and instrumentation

5. Gel-permeation chromatography

6. Ionex chromatography

7. Hydrophobic chromatography and reversed phase chromatography

8. Affinity chromatography

9. Recombinant proteins, production and troubleshooting

10. Thin layer chromatography, recovery and field of chromatography and determination of proteins concentration

11. Introduction to the electrophoretic methods

12. Types of elektrophoretic methods

13. General principles and distribution of imunechemical methods

14. Immunoaffinity separation of molecules

Registration requirements -
Last update: Kubová Petra Ing. (06.05.2019)

Biochemistry I

Course completion requirements -
Last update: Kubová Petra Ing. (06.05.2019)

Passing the exam consisting of a written and oral examination.

 
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