SubjectsSubjects(version: 949)
Course, academic year 2019/2020
Isolation and Analysis of Biomacromolecules - S320018
Title: Isolation and Analysis of Biomacromolecules
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2011 to 2020
Semester: winter
Points: winter s.:5
E-Credits: winter s.:5
Examination process: winter s.:
Hours per week, examination: winter s.:3/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Teaching methods: full-time
For type:  
Guarantor: Lipovová Petra doc. Ing. Ph.D.
Is interchangeable with: AB320010
Examination dates   Schedule   
Syllabus - Czech
Last update: KNOBLOCL (11.04.2012)

1. Introduction, cell disruption, filtration, ultrafiltration, dialysis

2. Precipitation, extraction, centrifugation (preparative, analytical), isolation of nucleic acid

3. Introduction to the chromatography, instrumentation and theory of chromatography

4. Gel chromatography

5. Ion exchange chromatography, chromatofocusation

6. Hydrophobic chromatography and reverse phase

7. Affinity chromatography

8. Recombinant proteins and their purification

9. Thin layer chromatography and recovery and field of chromatography

10. Introduction to the electrophoretic methods, SDS, native electrophoresis, blotting

11. 2D electrophoresis, isoelectric focusation

12. Pulse electrophoresis, capillary electrophoresis

13. Immobilisation of enzymes

Immobilisation of cell