SubjectsSubjects(version: 877)
Course, academic year 2020/2021
Analysis of Bioactive Compounds - AM323016
Title: Analysis of Bioactive Compounds
Guaranteed by: Department of Food Analysis and Nutrition (323)
Actual: from 2019
Semester: summer
Points: summer s.:4
E-Credits: summer s.:4
Examination process: summer s.:
Hours per week, examination: summer s.:2/1 C+Ex [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
For type: Master's (post-Bachelor)
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Stránská Milena doc. Ing. Ph.D.
Dvořáková Darina Ing. Ph.D.
Pulkrabová Jana prof. Ing. Ph.D.
Incompatibility : M323016
Interchangeability : M323016
N//Is incompatible with: M323016
Z//Is interchangeable with: M323016
This subject contains the following additional online materials
Last update: Pulkrabová Jana prof. Ing. Ph.D. (21.05.2018)
Within this course, principles and key applications of modern isolation, separation and detection techniques commonly used in the field of studies / characterization of biologically active compounds. Special attention is paid to (i) application of basic and advanced extraction techniques; (ii) effective separation strategy realized by gas / liquid chromatography; (iii) presentation of various instrumental techniques with a special focus on mass spectrometry and its presentation in case studies. In the final phase of course, practical aspects together with problems to be solved in a qualified manner in practice are summarized.
Aim of the course
Last update: Pulkrabová Jana prof. Ing. Ph.D. (21.05.2018)

Students will be able to understand:

How to make a qualified choice of suitable sample preparation strategy (i.e. extraction and clean-up technique) for determination of target analytes.

Utilization of separation techniques (gas/liquid chromatography) coupled with mass spectrometric detection for analysis of complex mixtures of natural compounds.

Basic characteristics of various ionization techniques and mass analyzers and their use for identification and quantification of analytes.

Application of non-target screening (metabolomic fingerprinting / profiling) followed by statistical processing of experimental data.

Last update: Pulkrabová Jana prof. Ing. Ph.D. (21.05.2018)

R: Otles S. (ed.): Handbook of Food Analysis Instruments. CRC Press, Boca Raton, USA, 2009, e-zdroj VŠCHT, ISBN 9781420045666.

R: W.M.A. Niessen: Current Practice of Gas Chromatography-Mass Spectrometry. Marcel Dekker, Inc., USA, 2001, ISBN 9780824704735.

R: W.M.A. Niessen: Liquid Chromatography-Mass Spectrometry. CRC Press, Boca Raton, USA, 2006, ISBN 978-0-8247-4082-5.

R: Watson J. T., Sparkman O. D.: Introduction to Mass Spectrometry. Wiley, Chichester, Velká Británie, 2007, ISBN 978-0-470-51634-8.

Learning resources
Last update: Pulkrabová Jana prof. Ing. Ph.D. (21.05.2018)

Techniques overview:

Compounds databases, mass spectra:

Requirements to the exam
Last update: Kubová Petra Ing. (16.04.2018)

Within the extent of the syllabus.

Last update: Pulkrabová Jana prof. Ing. Ph.D. (21.05.2018)

1. Introduction to the issue, classification of biologically active substances

2. General characteristics of the analytical procedure, performance characteristics and validation, basic steps in the analysis of biologically active substances of both natural and anthropogenic origin

3. Basic and advanced extraction techniques with a focus on their use in practice (case studies)

4. Gas chromatography (theory, injection techniques, classification of capillary columns, influence of various parameters on the separation process)

5. Gas chromatography (optimization of separation, two-dimensional GC, conventional detectors)

6. Liquid chromatography (theory, selection of chromatographic system)

7. Liquid chromatography (columns and classification of stationary phases, conventional detectors, optimization and influence of various parameters on the separation process)

8. Mass spectrometry (introduction and characterization of application potential, ionization techniques)

9. Mass spectrometry (mass analyzers, ion mobility, detection)

10. Quantification procedures and techniques, confirmation, matrix effects in GC and LC and possibilities of their reduction and compensation

11. Basic approaches in the analysis of biologically active substances (essential oils, vitamins, glycosides)

12. Basic approaches in the analysis of biologically active substances (biogenic amines, alkaloids, mycotoxins, environmental contaminants)

13. Non-target analysis (metabolomic fingerprinting and profiling, case studies)

14. Presentation of individual students works, discussion

Registration requirements
Last update: Kubová Petra Ing. (16.04.2018)

Analytical chemistry

Course completion requirements
Last update: Pulkrabová Jana prof. Ing. Ph.D. (21.05.2018)

To obtain the credit it is necessary to prepare a short presentation describing a case study in the field of analysis of biologically active substances. Assigned credit is a prerequisite for passing the exam, which consists of a written part.

Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 0,5 14
Účast na přednáškách 1 28
Práce na individuálním projektu 1 28
Příprava na zkoušku a její absolvování 1 28
Účast na seminářích 0,5 14
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