SubjectsSubjects(version: 891)
Course, academic year 2020/2021
Analysis of Bioactive Compounds - M323016
Title: Analýza biologicky aktivních látek
Guaranteed by: Department of Food Analysis and Nutrition (323)
Actual: from 2020
Semester: summer
Points: summer s.:4
E-Credits: summer s.:4
Examination process: summer s.:
Hours per week, examination: summer s.:2/1 C+Ex [hours/week]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type: Master's (post-Bachelor)
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Stránská Milena doc. Ing. Ph.D.
Dvořáková Darina Ing. Ph.D.
Incompatibility : AM323016
Interchangeability : AM323016, N323045
Is incompatible with: AM323016
Is interchangeable with: AM323016
This subject contains the following additional online materials
Annotation -
Last update: Dvořáková Darina Ing. Ph.D. (31.01.2018)
Within this course, principles and key applications of modern isolation, separation and detection techniques commonly used in the field of studies / characterization of biologically active compounds. Special attention is paid to (i) application of basic and advanced extraction techniques; (ii) effective separation strategy realized by gas / liquid chromatography; (iii) presentation of various instrumental techniques with a special focus on mass spectrometry and its presentation in case studies. In the final phase of course, practical aspects together with problems to be solved in a qualified manner in practice are summarized.
Aim of the course -
Last update: Fialová Jana (19.12.2017)

Students will be able to understand:

  • How to make a qualified choice of suitable sample preparation strategy (i.e. extraction and clean-up technique) for determination of target analytes.
  • Utilization of separation techniques (gas / liquid chromatography) coupled with mass spectrometric detection for analysis of complex mixtures of natural compounds.
  • Basic characteristics of various ionization techniques and mass analysers and their use for identification and quantification of analytes.
  • Application of non-target screening (metabolomic fingerprinting / profiling) followed by statistical processing of experimental data.
Literature -
Last update: Fialová Jana (19.12.2017)

R: Otles S. (ed.): Handbook of Food Analysis Instruments. CRC Press, Boca Raton, USA, 2009, e-zdroj VŠCHT, ISBN 9781420045666.

R: W.M.A. Niessen: Current Practice of Gas Chromatography-Mass Spectrometry. Marcel Dekker, Inc., USA, 2001, ISBN 9780824704735.

R: W.M.A. Niessen: Liquid Chromatography-Mass Spectrometry. CRC Press, Boca Raton, USA, 2006, ISBN 978-0-8247-4082-5.

R: Watson J. T., Sparkman O. D.: Introduction to Mass Spectrometry. Wiley, Chichester, Velká Británie, 2007, ISBN 978-0-470-51634-8.

Learning resources -
Last update: Dvořáková Darina Ing. Ph.D. (31.01.2018)

Techniques overview:

Compounds databases, mass spectra:

Requirements to the exam -
Last update: Vlčková Martina Ing. (30.01.2018)

Within the extent of the syllabus.

Syllabus -
Last update: Kubová Petra Ing. (16.02.2021)

1 Introduction to the issue, classification of biologically active substances

2 General characteristics of the analytical procedure, performance characteristics and validation, basic steps in the analysis of biologically active substances of both

natural and anthropogenic origin

3 Basic and advanced extraction techniques with a focus on their use in practice

4 Gas chromatography (theory, injection techniques, classification of capillary columns, influence of various parameters on the separation process, optimization of

separation, two-dimensional GC, conventional detectors)

5 Liquid chromatography (theory, selection of chromatographic system, columns and classification of stationary phases, conventional detectors, optimization and influence of

various parameters on the separation process)

6 Mass spectrometry (introduction and characterization of application potential, ionization techniques, mass analyzers, ion mobility, detection)

7 Analysis of volatile compounds (case studies)

8 Analysis of environmental contaminants by GC-MS (case studies)

9 Analysis of environmental contaminants by LC-MS (case studies)

10 Bioanalytical methods I

11 Bioanalytical methods II

12 Analysis of pesticides and mycotoxins

13 Analysis of vitamins and food additives

14 Presentation of individual students works, discussion, preparation for final test

Registration requirements -
Last update: Dvořáková Darina Ing. Ph.D. (31.01.2018)

Analytical chemistry

Course completion requirements -
Last update: Dvořáková Darina Ing. Ph.D. (31.01.2018)

To obtain the credit it is necessary to prepare a short presentation describing a case study in the field of analysis of biologically active substances. Assigned credit is a prerequisite for passing the exam, which consists of a written part.

Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 0,5 14
Účast na přednáškách 1 28
Práce na individuálním projektu 1 28
Příprava na zkoušku a její absolvování 1 28
Účast na seminářích 0,5 14
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