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Students will gain practically utilizable information on modern separation techniques, specifically liquid, gas, and supercritical chromatography, capillary electrophoresis, and mass spectrometry. Principles of the methods will be summarized. Then modern instruments will be shown and troubleshooting discusses. The practical case studies will be analyzed in detail and a potential of modern instrumentation for solution of complex analytical tasks will be demonstrated. Each student will receive a specific topic to cover in its bibliographic search and he/she will prepare presentation for the peers.
Last update: Pátková Vlasta (16.11.2018)
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The final assessment is based on the evaluation of written test exam and quality of individual bibliography search project including presentation. Last update: Pátková Vlasta (16.11.2018)
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A:High Performance Capillary Electrophoresis - D.N. Heiger, Agilent, 2000. A:Introduction to Modern Liquid Chromatography - book, L.R. Snyder, J.J. Kirkland and J.W. Dolan, Wiley, 2010, ISBN:978-0-470-16754-0. A:Gas Chromatography and Mass Spectrometry-A Practical Guide - book, O.D. Sparkman, Z.E. Penton, and F.G. Kitson, 2011, Elsevier, ISBN:978-0-12-373628-4. A:Introduction to Mass Spectrometry - book, J.T. Watson and O.D. Sparkman, Wiley, 2007, ISBN: 978-0-470-51634-8. Last update: Pátková Vlasta (16.11.2018)
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Lectures, seminars, practical demonstrations, presentations. Last update: Pátková Vlasta (16.11.2018)
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1) Principles of gas (GC), liquid (LC) and supercritical fluid chromatography 2) Principles of capillary electrophoresis (CE) a mass spectrometry (MS) 3) Multidimensional techniques of GC 4) GC troubleshooting 5) Practical demonstration of instrumentation and selected case studies in GC 6) Multidimensional techniques of LC 7) LC troubleshooting 8) Practical demonstration of instrumentation and selected case studies in LC 9) Case studies in the forensic analysis 10) Case studies in the analysis of pharmaceuticals 11) Case studies in the analysis of drugs 12) Modern chiral separations in the field of CE 13) Practical demonstration of instrumentation and selected case studies in CE 14) Presentation of bibliography search projects Last update: Pátková Vlasta (16.11.2018)
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Students will gain: new practically applicable skills in the area of up-to-date separation methods knowledge how troubleshoot a modern instrumentation insight to the analysis of complex samples utilizing advanced separation methods practical experience with moder instrumentation abilities related with elaboration of a bibliographical search and its prezentation Last update: Pátková Vlasta (16.11.2018)
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Separation methods - graduate level. Last update: Pátková Vlasta (16.11.2018)
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Analytical chemistry - bachelor's level. Last update: Pátková Vlasta (16.11.2018)
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