SubjectsSubjects(version: 923)
Course, academic year 2021/2022
Doplnit ! - P402005
Title: Separační metody pro doktorandy
Guaranteed by: Department of Analytical Chemistry (402)
Faculty: Faculty of Chemical Engineering
Actual: from 2019 to 2021
Semester: summer
Points: summer s.:0
E-Credits: summer s.:0
Examination process: summer s.:
Hours per week, examination: summer s.:2/1, other [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type: doctoral
Additional information:
Note: course is intended for doctoral students only
can be fulfilled in the future
Guarantor: Sýkora David doc. RNDr. Dr.
Řezanka Pavel doc. RNDr. Ing. Ph.D.
Interchangeability : D402010
Is interchangeable with: AP402005
This subject contains the following additional online materials
Annotation -
Last update: Sýkora David doc. RNDr. Dr. (03.05.2022)
Students will gain new information on modern separation techniques, specifically liquid, gas, and supercritical chromatography, capillary electrophoresis, and mass spectrometry. Principles of the methods will be summarized. Potential of modern instrumentation for solution of complex analytical tasks will be demonstrated with the emphasis on multidimensional separations. Each student will receive a specific topic to cover in its bibliographic search and he/she will prepare presentation for the peers.
Aim of the course -
Last update: Sýkora David doc. RNDr. Dr. (03.05.2022)

Students will gain:

new knowledge in the area of up-to-date separation methods

insight to the analysis of complex samples utilizing advanced separation methods

abilities related with elaboration of a bibliographical search and its prezentation

Literature -
Last update: Sýkora David doc. RNDr. Dr. (03.05.2022)

A:Two-Dimensional Liquid Chromatography - firemní příručka. P.W. Carr, D.R. Stoll, Agilent, 2016.

A:High Performance Capillary Electrophoresis - D.N. Heiger, Agilent, 2000.

A:Introduction to Modern Liquid Chromatography - book, L.R. Snyder, J.J. Kirkland and J.W. Dolan, Wiley, 2010, ISBN:978-0-470-16754-0.

A:Gas Chromatography and Mass Spectrometry-A Practical Guide - book, O.D. Sparkman, Z.E. Penton, and F.G. Kitson, 2011, Elsevier, ISBN:978-0-12-373628-4.

A:Introduction to Mass Spectrometry - book, J.T. Watson and O.D. Sparkman, Wiley, 2007, ISBN: 978-0-470-51634-8.

Teaching methods -
Last update: Sýkora David doc. RNDr. Dr. (31.08.2018)

Lectures, seminars, practical demonstrations, presentations.

Syllabus -
Last update: Sýkora David doc. RNDr. Dr. (03.05.2022)

1) Principles of gas (GC), liquid (LC) and supercritical fluid chromatography

2) Principles of capillary electrophoresis (CE) a mass spectrometry (MS)

3) Multidimensional techniques of GC I

4) Multidimensional techniques of GC II

5) Multidimensional techniques of GC III

6) Multidimensional techniques of LC I

7) Multidimensional techniques of LC II

8) Multidimensional techniques of LC III

9) Case studies in the forensic analysis

10) Case studies in the analysis of pharmaceuticals

11) Modern chiral separations in the field of CE

12) Chiral selectors in CE

13) Presentation of bibliography search projects I

14) Presentation of bibliography search projects II

Entry requirements -
Last update: Sýkora David doc. RNDr. Dr. (31.08.2018)

Separation methods - graduate level.

Registration requirements -
Last update: Sýkora David doc. RNDr. Dr. (31.08.2018)

Analytical chemistry - bachelor's level.

Course completion requirements -
Last update: Sýkora David doc. RNDr. Dr. (01.09.2018)

The final assessment is based on the evaluation of written test exam and quality of individual bibliography search project including presentation.