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Last update: Kubová Petra Ing. (04.01.2018)
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Last update: Kubová Petra Ing. (04.01.2018)
Students will be able to:
Independently conduct and properly record particular experiment. Isolate specific DNA molecules from cells and conduct their analysis through restriction digestion and nucleotide sequencing.
Conduct and interpret basic of electrophoretic DNA and protein analyses.
Understand the importance specific working procedures in molecular biology laboratory and will be knowledgeable in options allowing optimizing selected parameters of the experiments introduced during the course. |
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Last update: Kubová Petra Ing. (04.01.2018)
R: Ruml T., Rumlová M., Pačes V., Genové inženýrství, VŠCHT Praha, 2002, 9788070804995 |
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Last update: Kubová Petra Ing. (04.01.2018)
manufacturers´s protocols and instructions and tips at http://www.protocol-online.org/ |
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Last update: Kubová Petra Ing. (04.01.2018)
1. Operational safety in laboratory, good laboratory practice and recording an experiment
2. Introduction to course organization and the proper use of specific labware and chemicals
3. Small-scale izolation of plasmid DNA
4. Restriction digestion of plasmid DNA
5. Electrophoretic analysis of the fragments of digested plasmid
6. Polymerase chain reaction (PCR)
7. Electrophoretic analysis to verify a PCR amplicon correct
8. DNA sequencing reaction
9. Analysis of sequencing products and reading the DNA sequence
10. Preparation of an extract from cells producing a recombinant protein
11. Electrophoretic separation of cell-free extract and of isolated recombinant protein
12. Recording and interpretation of the electrophoreogram of separated proteins
13. Discussion about optional experimental settings and their impact on results
14. Completion of final report |
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Last update: Kubová Petra Ing. (04.01.2018)
none |
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Last update: Uhlík Ondřej prof. Ing. Bc. Ph.D. (05.03.2018)
Passsing oral exam |