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The course focuses on understanding the principles of purification techniques for the isolation of biomolecules. The aim is to teach students how to design a proper procedure for the purification of biomolecules target and characterize it. This knowledge can be used in many fields.
Last update: Lipovová Petra (24.01.2018)
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Passing the exam consisting of a written and oral examination. Last update: Lipovová Petra (24.01.2018)
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R: Janson J.C.: Protein Purification, Wiley, New York, 1998, ISBN 0471186260 R: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New York, 2004, ISBN 0471544477 D: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New Jersey, 2016, ISBN 978-1-118-30254-5 D: Manz A., Dittrich P.S., Pamme N., Iossifidis D.: Bioanalytical Chemistry 2nd Edition, London, 2015, ISBN 978-1-78326-671-5 Last update: Lipovová Petra (09.08.2022)
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1. Introduction - source of proteins, dezintegration, membrane process 2. Precipitation, extraction, and centrifugation 3. Materials for chromatographic and eletrophoretic methods 4. The basic division and instrumentation 5. Gel-permeation chromatography 6. Ionex chromatography 7. Hydrophobic chromatography and reversed phase chromatography 8. Affinity chromatography 9. Recombinant proteins, production and troubleshooting 10. Thin layer chromatography, recovery and field of chromatography and determination of proteins concentration 11. Introduction to the electrophoretic methods 12. Types of elektrophoretic methods 13. General principles and distribution of imunechemical methods 14. Immunoaffinity separation of molecules Last update: Lipovová Petra (15.01.2018)
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e-learning.vscht.cz Last update: Lipovová Petra (15.01.2018)
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Students will be able to: Understand the principles of membrane, chromatographic, electrophoretic techniques, and immunoaffinity methods. Propose a procedure to optimize protein purification.
Last update: Lipovová Petra (15.01.2018)
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Biochemistry I Last update: Lipovová Petra (24.01.2018)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Obhajoba individuálního projektu | 0.5 | 14 | ||
Účast na přednáškách | 1 | 28 | ||
Práce na individuálním projektu | 0.5 | 14 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
3 / 3 | 84 / 84 |
Coursework assessment | |
Form | Significance |
Report from individual projects | 20 |
Examination test | 40 |
Oral examination | 40 |