SubjectsSubjects(version: 941)
Course, academic year 2021/2022
Isolation and Characterisation of Biomacromolecules - B320010
Title: Izolace a charakterizace biomakromolekul
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2021 to 2021
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
For type: Bachelor's
Additional information:
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Lipovová Petra doc. Ing. Ph.D.
Holubová Barbora doc. Ing. Ph.D.
Interchangeability : AB320010, N320018
Is interchangeable with: AB320010
This subject contains the following additional online materials
Annotation -
Last update: Lipovová Petra doc. Ing. Ph.D. (24.01.2018)
The course focuses on understanding the principles of purification techniques for the isolation of biomolecules. The aim is to teach students how to design a proper procedure for the purification of biomolecules target and characterize it. This knowledge can be used in many fields.
Aim of the course -
Last update: Lipovová Petra doc. Ing. Ph.D. (15.01.2018)

Students will be able to:

Understand the principles of membrane, chromatographic, electrophoretic techniques, and immunoaffinity methods.

Propose a procedure to optimize protein purification.

Literature -
Last update: Lipovová Petra doc. Ing. Ph.D. (09.08.2022)

R: Janson J.C.: Protein Purification, Wiley, New York, 1998, ISBN 0471186260

R: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New York, 2004, ISBN 0471544477

D: Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New Jersey, 2016, ISBN 978-1-118-30254-5

D: Manz A., Dittrich P.S., Pamme N., Iossifidis D.: Bioanalytical Chemistry 2nd Edition, London, 2015, ISBN 978-1-78326-671-5

Learning resources -
Last update: Lipovová Petra doc. Ing. Ph.D. (15.01.2018)

Syllabus -
Last update: Lipovová Petra doc. Ing. Ph.D. (15.01.2018)

1. Introduction - source of proteins, dezintegration, membrane process

2. Precipitation, extraction, and centrifugation

3. Materials for chromatographic and eletrophoretic methods

4. The basic division and instrumentation

5. Gel-permeation chromatography

6. Ionex chromatography

7. Hydrophobic chromatography and reversed phase chromatography

8. Affinity chromatography

9. Recombinant proteins, production and troubleshooting

10. Thin layer chromatography, recovery and field of chromatography and determination of proteins concentration

11. Introduction to the electrophoretic methods

12. Types of elektrophoretic methods

13. General principles and distribution of imunechemical methods

14. Immunoaffinity separation of molecules

Registration requirements -
Last update: Lipovová Petra doc. Ing. Ph.D. (24.01.2018)

Biochemistry I

Course completion requirements -
Last update: Lipovová Petra doc. Ing. Ph.D. (24.01.2018)

Passing the exam consisting of a written and oral examination.

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 2 56
3 / 3 84 / 84