SubjectsSubjects(version: 955)
Course, academic year 2023/2024
Laboratory of biological materials analysis - B320019
Title: Laboratoř analýzy biologických materiálů
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2022
Semester: winter
Points: winter s.:2
E-Credits: winter s.:2
Examination process: winter s.:
Hours per week, examination: winter s.:0/2, MC [HT]
Capacity: unknown / 32 (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: Lipov Jan doc. Ing. Ph.D.
Class: Základní laboratoře
Interchangeability : N320085
This subject contains the following additional online materials
Annotation -
The aim of the subject is to introduce the molecular biology techniques of forensic analysis of common biological samples. It deals with isolation of nucleic acids, evaluation of its amount and integrity. The key part is the introduction to the techniques of forensic human identification with overlap to laboratory practice.
Last update: Kubová Petra (18.12.2017)
Aim of the course -

Students will be able to:

understand the basics of DNA and RNA work

isolate nucleic acids from common biological sources

understand the basics of nucleic acid analysis by restriction cleavage

understand the basics of amplification using PCR

understand the basics of quantification of DNA using real-time PCR and digital PCR

understand the basics of human identification using RFPL, VNTR, STR, SNP

Last update: Lipov Jan (23.01.2018)
Literature -

A: Butler J.M., Fundamentals of Forensic DNA Typing, Elsevier Publishing 2009, 978-0123749994

Last update: Kubová Petra (18.12.2017)
Learning resources -


Last update: Kubová Petra (18.12.2017)
Syllabus -

1. Introduction, safety in molecular biology laboratory

2. Basics of work with biological material, namely DNA and RNA

3. Isolation of DNA from buccal swab

4. DNA integrity assessment by electrophoresis, measurement of concentration using spectrophotometry

5. Polymerase chain reaction (PCR)

6. VNTR analysis, lenght of the fragments, determination of number of repetitions

7. mt DNA analysis - advantages and restrictions

8. RFLP analysis - cleavage of DNA by restriction endonucleases

9. Electrophoretic separation and analysis of rectriction fragments

10. Nucleic acid quantification using real-time PCR

11. Fundamentals of optimalization of polymerase reactions and primer selection, factors affecting specifity and yield

12. Human identification based on short tandem repetition (STR) analysis I - multiplex PCR

13. Human identification based on short tandem repetition (STR) analysis II - capillary analysis of DNA amplicons

14. Basics of data evaluation, knowledge and results evaluation

Last update: Lipov Jan (14.02.2018)
Registration requirements -


Last update: Kubová Petra (18.12.2017)
Course completion requirements -

Active presence on all days of the course.

At least 50% from all the tests.

Individual elaboration of summary protocol.

At least "E" from the protocol.

Last update: Lipov Jan (25.01.2018)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
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