SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Laboratory of biological materials analysis - N320085
Title: Laboratoř analýzy biologických materiálů
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2017
Semester: winter
Points: winter s.:2
E-Credits: winter s.:2
Examination process: winter s.:
Hours per week, examination: winter s.:0/2 MC [hours/week]
Capacity: unknown / 48 (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Guarantor: Lipov Jan Ing. Ph.D.
Jablonská Eva Ing. Ph.D.
This subject contains the following additional online materials
Annotation -
Last update: Lipov Jan Ing. Ph.D. (08.08.2013)
The aim of the subject is to introduce the molecular biology techniques of forensic analysis of common biological samples. It deals with isolation of nucleic acids, evaluation of its amount and integrity. The key part is the introduction to the techniques of forensic human identification with overlap to laboratory practice.
Aim of the course -
Last update: Jablonská Eva Ing. Ph.D. (06.09.2019)

Students will be able to:

understand the basics of DNA and RNA work

isolate DNA from common sources

understand the basics of nucleic acid analysis by restriction cleavage (RFLP)

understand the basics of quantification of DNA using real-time PCR

understand the basics of human identification using VNTR and STR polymorphism

understand the basics of DNA sequencing and fragment analysis

Literature -
Last update: Lipov Jan Ing. Ph.D. (08.08.2013)

A: Butler J.M., Fundamentals of Forensic DNA Typing, Elsevier Publishing 2009, 978-0123749994

Learning resources -
Last update: Lipov Jan Ing. Ph.D. (30.08.2013)

none

Syllabus -
Last update: Jablonská Eva Ing. Ph.D. (06.09.2019)

1. Introduction, safety in molecular biology laboratory

2. Basics of work with biological material, namely DNA

3. Isolation of DNA from buccal swab, quality and quantity assessment

4. Polymerase chain reaction (PCR) - amplification of tandem repeats (locus D1S80)

5. Electrophoretic separation of amplicons

6. Determination of number of repeats (locus D1S80) and random match probability (RMP)

7. Amplification of mitochondrial DNA (mtDNA) using PCR

8. RFLP analysis of mtDNA - cleavage of DNA by restriction endonucleases

9. Electrophoretic separation and analysis of rectriction fragments

10. Nucleic acid quantification using quantitative PCR (qPCR)

11. Multiplex PCR of short tandem repeats (STR)

12. Fragment analysis of amplicons using capillary electrophoresis

13. Evaluation of STR profiles, determination of RMP, analysis of mixed profiles

14. Basics of paternity evaluation based on STR

Registration requirements -
Last update: Lipov Jan Ing. Ph.D. (30.08.2013)

none

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na zkoušku a její absolvování 1 28
2 / 2 56 / 56
Coursework assessment
Form Significance
Report from individual projects 50
Examination test 50

 
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