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The aim of the subject is to introduce the molecular biology techniques of forensic analysis of common biological samples. It deals with isolation of nucleic acids, evaluation of its amount and integrity. The key part is the introduction to the techniques of forensic human identification with overlap to laboratory practice.
Last update: Kubová Petra (18.12.2017)
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Students will be able to: understand the basics of DNA and RNA work isolate nucleic acids from common biological sources understand the basics of nucleic acid analysis by restriction cleavage understand the basics of amplification using PCR understand the basics of quantification of DNA using real-time PCR and digital PCR understand the basics of human identification using RFPL, VNTR, STR, SNP Last update: Lipov Jan (23.01.2018)
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Active presence on all days of the course. At least 50% from all the tests. Individual elaboration of summary protocol. At least "E" from the protocol. Last update: Lipov Jan (25.01.2018)
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A: Butler J.M., Fundamentals of Forensic DNA Typing, Elsevier Publishing 2009, 978-0123749994 Last update: Kubová Petra (18.12.2017)
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1. Introduction, safety in molecular biology laboratory 2. Basics of work with biological material, namely DNA and RNA 3. Isolation of DNA from buccal swab 4. DNA integrity assessment by electrophoresis, measurement of concentration using spectrophotometry 5. Polymerase chain reaction (PCR) 6. VNTR analysis, lenght of the fragments, determination of number of repetitions 7. mt DNA analysis - advantages and restrictions 8. RFLP analysis - cleavage of DNA by restriction endonucleases 9. Electrophoretic separation and analysis of rectriction fragments 10. Nucleic acid quantification using real-time PCR 11. Fundamentals of optimalization of polymerase reactions and primer selection, factors affecting specifity and yield 12. Human identification based on short tandem repetition (STR) analysis I - multiplex PCR 13. Human identification based on short tandem repetition (STR) analysis II - capillary analysis of DNA amplicons 14. Basics of data evaluation, knowledge and results evaluation Last update: Lipov Jan (14.02.2018)
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none Last update: Kubová Petra (18.12.2017)
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none Last update: Kubová Petra (18.12.2017)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 1 | 28 | ||
2 / 2 | 56 / 56 |