SubjectsSubjects(version: 954)
Course, academic year 2023/2024
Microorganisms in Polluted Environment - M240015
Title: Mikroorganismy v kontaminovaném prostředí
Guaranteed by: Department of Environmental Chemistry (240)
Faculty: Faculty of Environmental Technology
Actual: from 2023
Semester: summer
Points: summer s.:5
E-Credits: summer s.:5
Examination process: summer s.:
Hours per week, examination: summer s.:2/2, C+Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Chumchalová Jana Dr. Ing.
Class: Základní laboratoře
Interchangeability : N240027
Annotation -
The aim of the course is to teach students the part of environmental microbiology concerning the occurrence of contaminants in environment and their biodegradation. Emphasis will be placed on the main principles of biodegradation and bioremediation. In addition, the course focuses on the various types of environmental contamination with harmful substances (e.g. contamination by petroleum products), microbial decomposition of these harmful substances in the environment and methods detecting degradation process. Part of the subject will be directed to the laboratory experiments. Students will obtain theme of a real problem. They have to suggest the procedure, realize it in the laboratory and discuss obtained results.
Last update: Chumchalová Jana (09.02.2018)
Aim of the course -

Students will be able to:

determine various types of environmental contamination by harmful substances,

know the possible microbiological decomposition of harmful substances,

limitations of microbiological degradation of contaminants.

Last update: Chumchalová Jana (09.02.2018)
Literature -

Pepper I.L., Gerba G.P., Gentry T.J. Environmental Microbiology. 3. vyd. Elsevier, 2015. ISBN 9780123946263.

Palmisano A.C., Barlaz A.M. Microbiology of solid waste. CRC Press,1996. ISBN 0849383617.

Plíva P. a kol. Kompostování a kompostárna. Praha, 2016 Profi Press. ISBN 9788086726748.

Last update: Chumchalová Jana (09.02.2018)
Learning resources -

Horáková D.: Bioremediace. 2006, skriptum Masarykova univerzita,

Chumchalová J., Kubal M.: Praktická cvičení z environmentální mikrobiologie, VŠCHT Praha, 2017.

Miniatlas mikroorganismů.

Last update: Chumchalová Jana (09.02.2018)
Requirements to the exam -

Rules to grant credit: student will attend laboratories and deliver report from laboratory praxis in term.

Rules to get exam: student will consist within syllabus of a written part and then listener will be interviewed orally.

Last update: Chumchalová Jana (09.02.2018)
Syllabus -

1. Characterization of microorganism environment, microbial profile of environment, interactions between microorganisms, microorganisms and plants.

2. General principles of biodegradation processes. The effect of environment on biodegradation (e.g. redox potential and pH environment).

3. Bioavailability and bioaccessibility of pollutants.

4. Petroleum products and petroleum-degrading microorganisms (principles and limits of microbial degradation, detection of microbial degradation).

5. Chlorinated hydrocarbons and microorganisms degrading chlorinated hydrocarbons (principles and limits of microbial degradation, detection of microbial degradation).

6. Persistent organic pollutants and microorganisms degrading persistent organic pollutants (principles and limits of microbial degradation, detection of microbial degradation).

7. Inorganic pollutants, radionuclides, heavy metals.

8. Landfill, microorganisms in landfill sites and microbiological processes during waste decomposition.

9. Composting, microorganisms in compost and microbiological processes during composting.

10. Theoretical preparation of experiment for laboratory testing.

11. Laboratory exercises – Preparation of sterile aids, dilution solutions, cultivation and diagnostic media.

12. Laboratory exercises – Determination of total degraders.

13. Laboratory exercises – Determination of degradation process.

14. Laboratory exercises – Identification of total degraders by Biolog system.

Last update: Chumchalová Jana (09.02.2018)
Registration requirements -

Basic knowledge from biology and microscopic techniques.

Last update: Chumchalová Jana (09.02.2018)
Course completion requirements -

Students must to undergo all laboratory tests, to write protocol under rules and to surrend it in term, to write a test and to obtain a minimum 50% of points and pass an oral examination.

Last update: Chumchalová Jana (09.02.2018)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 2 56
Příprava na zkoušku a její absolvování 2 56
5 / 5 140 / 140