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The aim of the course is to teach students the part of environmental microbiology concerning the occurrence of contaminants in environment and their biodegradation. Emphasis will be placed on the main principles of biodegradation and bioremediation. In addition, the course focuses on the various types of environmental contamination with harmful substances (e.g. contamination by petroleum products), microbial decomposition of these harmful substances in the environment and methods detecting degradation process. Part of the subject will be directed to the laboratory experiments. Students will obtain theme of a real problem. They have to suggest the procedure, realize it in the laboratory, present and discuss obtained results.
Last update: Chumchalová Jana (28.08.2024)
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Students will be able to: determine various types of environmental contamination by harmful substances, know the possible microbiological decomposition of harmful substances, limitations of microbiological degradation of contaminants. Last update: Chumchalová Jana (09.02.2018)
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Students must to undergo all laboratory tests, to write protocol under rules and to surrend it in term and pass an oral examination in the form of presentation. Last update: Chumchalová Jana (28.08.2024)
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Recommended:
Optional:
Last update: Chumchalová Jana (28.08.2024)
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Rules to grant credit: student will attend laboratories and deliver report from laboratory praxis in term. Rules to get exam: student will prepare a presentation within the scope of laboratory assignment, where listener will be questioned orally.
Last update: Chumchalová Jana (28.08.2024)
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1. Characterization of microorganism environment, microbial profile of environment, interactions between microorganisms, microorganisms and plants. 2. General principles of biodegradation processes. The effect of environment on biodegradation (e.g. redox potential and pH environment). 3. Bioavailability and bioaccessibility of pollutants. 4. Petroleum products and petroleum-degrading microorganisms (principles and limits of microbial degradation, detection of microbial degradation). 5. Chlorinated hydrocarbons and microorganisms degrading chlorinated hydrocarbons (principles and limits of microbial degradation, detection of microbial degradation). 6. Persistent organic pollutants and microorganisms degrading persistent organic pollutants (principles and limits of microbial degradation, detection of microbial degradation). 7. Inorganic pollutants, radionuclides, heavy metals. 8. Design of a biodegradation experiment for laboratory testing - introduction to the problem. 9. Theoretical preparation of a biodegradation experiment - design for laboratory testing. 10. Theoretical preparation of biodegradation experiment for laboratory testing - method of evaluating degradation. 11. Laboratory exercises – Preparation of sterile aids, dilution solutions, cultivation and diagnostic media. 12. Laboratory exercises – Determination of total degraders. 13. Laboratory exercises – Determination of degradation process. 14. Laboratory exercises – Evaluation of contaminant degradation process. Last update: Chumchalová Jana (28.08.2024)
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Horáková D.: Bioremediace. 2006, skriptum Masarykova univerzita, http://is.muni.cz/do/1499/el/estud/prif/ps06/3062932/bioremediace_FRVS_2006-2.pdf Chumchalová J., Kubal M., Šír M.: Praktická cvičení z environmentální mikrobiologie, VŠCHT Praha, aktualizovaný učební text Chumchalová J., Němec M., Kotoučková L., Páčová Z., Savická D., Kubátová A., Patáková P.: Miniatlas mikroorganismů. http://old.vscht.cz/obsah/fakulty/fpbt/ostatni/miniatlas/index.htm Last update: Chumchalová Jana (28.08.2024)
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Basic knowledge from biology and microscopic techniques. Last update: Chumchalová Jana (09.02.2018)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 2 | 56 | ||
Příprava na zkoušku a její absolvování | 2 | 56 | ||
5 / 5 | 140 / 140 |