SubjectsSubjects(version: 888)
Course, academic year 2020/2021
  
Fundamentals of Immunochemistry and Laboratory of Immunochemistry - M320004
Title: Základy imunochemie a laboratoř imunochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2020
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:0/4 MC [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Guarantor: Hochel Igor doc. Ing. CSc.
Interchangeability : N320026
Annotation -
Last update: Hochel Igor doc. Ing. CSc. (13.12.2018)
The course is focused on mastering the procedures of various immunochemical assays.
Aim of the course -
Last update: Hladíková Jana (13.12.2017)

Students will be able to:

Design the working procedures and to apply immunochmical methods in food and clinical control and in environmental testing.

Literature -
Last update: Hladíková Jana (13.12.2017)

R: Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418

Learning resources -
Last update: Hladíková Jana (13.12.2017)

Other study aids:

protocols: http://biomikro.vscht.cz/vyuka/?Predmet=fm

Syllabus -
Last update: Hochel Igor doc. Ing. CSc. (31.01.2018)

1. Laboratory rules, occupational health and safety

2. Immunoprecipitation in liquid phase

3. Double diffusion according to Ouchterlony

4. Radial immunodiffusion according to podle Mancini

5. Electrosyneresis

6. Immunoelectrodiffusion according to Laurell

7. Indirect competitive ELISA

8. Non-competitive ELISA

9. GLISA Rapid test

10. SDS-PAGE of proteins, blotting and immunochemical detection of analytes

11. Immunoagglutination

Registration requirements -
Last update: Hladíková Jana (13.12.2017)

Biochemistry, Microbiology

Course completion requirements -
Last update: Hochel Igor doc. Ing. CSc. (24.01.2018)

presentation of results and completed written exams

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 2 56
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,5 14
Příprava na zkoušku a její absolvování 0,5 14
3 / 3 84 / 84
 
VŠCHT Praha