Immunochemistry and Laboratory of Immunochemistry - M320004
Title: Imunochemie a laboratoř imunochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2023
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:1/3, MC [HT]
Capacity: unlimited / 56 (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Level:  
Guarantor: Holubová Barbora doc. Ing. Ph.D.
Class: Základní laboratoře
Interchangeability : N320026
Examination dates   
This subject contains the following additional online materials
Annotation -
The course is focused on mastering the procedures of various immunochemical assays.
Last update: Hochel Igor (13.12.2018)
Aim of the course -

Students will be able to:

Design the working procedures and to apply immunochmical methods in food and clinical control and in environmental testing.

Last update: Hladíková Jana (13.12.2017)
Course completion requirements -

presentation of results and completed written exams

Last update: Hochel Igor (24.01.2018)
Literature -

Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418 (z)

Fukal L., Holubová B.: Imunochemie a imunoanalýza. Praha 2007, ISBN 978-80-239-8903-8 (d)

Last update: Holubová Barbora (15.09.2023)
Syllabus -

The lectures will focus on the basic functions of the individual components of the immune system; characteristics related to the structure and properties of antigens and antibodies; possibilities of antibody preparation; parameters of antigen-antibody interaction in vitro; haptens and immunoassay; details of procedures of basic immunochemical methods (precipitation-non-precipitation, label-free with label; enzyme immunoassay and differences in its variations; immunoblot, immunochromatographic assay, immunohistochemical applications, etc. In subsequent laboratory exercises, the theoretical foundations acquired in the lectures will be combined with the practical implementation of selected laboratory techniques (ELISA, ICT, immunoblot).

Last update: Holubová Barbora (15.09.2023)
Learning resources -

Lectures and manuals in electronic form published on e-learning.vscht.cz.

Last update: Holubová Barbora (15.09.2023)
Registration requirements -

Biochemistry, Microbiology

Last update: Hladíková Jana (13.12.2017)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 2 56
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0.5 14
Příprava na zkoušku a její absolvování 0.5 14
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