SubjectsSubjects(version: 877)
Course, academic year 2020/2021
  
Molecular mechanisms of bacterial virulence - M320024
Title: Molekulární mechanismy bakteriální virulence
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2019
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0 Ex [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Guarantor: Šebo Peter prof. Ing. CSc.
Osička Radim Ing. Ph.D.
Kamanová Jana RNDr. Ph.D.
Annotation -
Last update: Hladíková Jana (13.12.2017)
The course deals with molecular mechanisms of host-pathogen interactions. Upon recapitulation of the basic mechanisms of mammalian immune system function, the lectures will focus on genetic basis and regulation mechanisms underlying virulence of bacterial pathogens. Molecular details of action of major virulence factors, such as adhesins, toxins, complement resistance and immunomodulatory substances will be discussed. Action of virulence factors will be discussed from the systemic context of the entire host organism down to the level of biology and physiology of cells of the immune system. Principles of host-pathogen interactions will then be analyzed using selected examples of major human pathogens and bacterial infections.
Aim of the course -
Last update: Hladíková Jana (13.12.2017)

Students will be able to:

  • use the acquired knowledge in development of novel methods for detection of pathogens and contaminants in foods and for the purpose of clinical diagnostics of infectious diseases.
  • apply the acquired knowledge for development of novel and more efficient vaccines and for characterization of immune responses.
  • characterize the most important bacterial pathogens and food contaminants of concern to man and animals.

Literature -
Last update: Šebo Peter prof. Ing. CSc. (19.01.2018)

Z: Brenda A. Wilson,‎ Abigail A. Salyers,‎ Dixie D. Whitt,‎ Malcolm E. Winkler: Bacterial Pathogenesis: A Molecular Approach, 3rd edition 2010 ASM Press, ISBN-13: 978-0632037759

Z:Kenneth Murphy, Casey Weaver: Janeway's Immunobiology, Ninth Edition (Immunobiology: The Immune System (Janeway), 2017, Garland Science, ISBN-13: 978-0815345053

D:Goering R., Dockrell H., Roitt I., et al.: Mims' Medical Microbiology, 2012, ELSEVIER, ISBN 9780808924401, 9780702056635.

Learning resources -
Last update: Šebo Peter prof. Ing. CSc. (22.01.2018)

http://l125lsx.mbu.cas.cz/Lab125/index.php/component/jdownloads/category/2-bacterial-pathogenesis?Itemid=479

Syllabus -
Last update: Šebo Peter prof. Ing. CSc. (19.01.2018)

1. Molecular mechanisms of anti-infectious immunity

2. Classes and mechanisms of action of bacterial virulence factors

3. Genetic equipment and gene expression regulation of bacterial pathogens

4. Experimental/animal systems for identification of virulence genes and for analysis of host-pathogen interactions

5. Protective mechanisms enabling bacteria to overcome innate and fool adaptive immunity

6. Cell biology and mechanisms of bacterial toxin action, intracellular bacterial pathogens

7. Vaccines as tools for protection from infection, modulation of host response and immunotherapy

8. Antibiotics as anti-infectious therapeutics and resistance to antibiotics

9. Intestinal microflora, commensals - the human microbiome

10. Most studied bacterial pathogens - an introductory overview:

Corynebacterium diphteriae, Streptococcus pyogenes a Staphylococcus aureus

11. Most studied bacterial pathogens:

Clostridium botulinum/perfringens/tetani, B. cereus, Helicobacter pylori

12. Most studied bacterial pathogens:

Ricketsia, Fracisella, Coxiella, Yersinia, Salmonella, Shigella,

13. Most studied bacterial pathogens:

Campylobacter jejuni, Vibrio cholerae, Escherichia coli - EPEC, ETEC, UPEC, NMEC

14. Most studied bacterial pathogens:

Bordetella, Mycobacterium, Neisseria, Listeria, Borelia, Mycoplasma, Chlamydia

Registration requirements -
Last update: Šebo Peter prof. Ing. CSc. (30.01.2018)

Biochemistry, Microbiology and Molecular biology

Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 0,7 20
Účast na přednáškách 1 28
Práce na individuálním projektu 0,6 16
Příprava na zkoušku a její absolvování 0,7 20
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