SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Molecular mechanisms of bacterial virulence - N320088
Title: Molekulární mechanismy bakteriální virulence
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2013
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Guarantor: Šebo Peter prof. Ing. CSc.
Is interchangeable with: M320024
Annotation -
Last update: Šmídová Ludmila (09.07.2013)
The course deals with molecular mechanisms of host-pathogen interactions. Upon recapitulation of the basic mechanisms of mammalian immune system function, the lectures will focus on genetic basis and regulation mechanisms underlying virulence of bacterial pathogens. Molecular details of action of major virulence factors, such as adhesins, toxins, complement resistance and immunomodulatory substances will be discussed. Action of virulence factors will be discussed from the systemic context of the entire host organism down to the level of biology and physiology of cells of the immune system. Principles of host-pathogen interactions will then be analyzed using selected examples of major human pathogens and bacterial infections.
Aim of the course -
Last update: Šmídová Ludmila (09.07.2013)

Students will be able to:

  • use the acquired knowledge in development of novel methods for detection of pathogens and contaminants in foods and for the purpose of clinical diagnostics of infectious diseases.
  • apply the acquired knowledge for development of novel and more efficient vaccines and for characterization of immune responses.
  • characterize the most important bacterial pathogens and food contaminants of concern to man and animals.

Literature -
Last update: Šmídová Ludmila (09.07.2013)

R:Salyers A.A., Whitt D.D.: Bacterial Pathogenesis: A Molecular Approach, 2007 ASM Press, 978-1555811716

R:Janeway's Immunobiology, Seventh Edition (Immunobiology: The Immune System (Janeway), 2007, Garland Science, 978-0815341239

A:Goering R., Dockrell H., Roitt I., et al.: Mims' Medical Microbiology, 2007, ELSEVIER, 9780323044752

Learning resources -
Last update: Šmídová Ludmila (09.07.2013)

https://l125lsx.mbu.cas.cz/BacPatho/

Syllabus -
Last update: Šmídová Ludmila (10.07.2013)

1. Molecular mechanisms of anti-infectious immunity

2. Classes and mechanisms of action of bacterial virulence factors

3. Genetic equipment and gene expression regulation of bacterial pathogens

4. Experimental/animal systems for identification of virulence genes and for analysis of host-pathogen interactions

5. Protective mechanisms enabling bacteria to overcome innate and fool adaptive immunity

6. Cell biology and mechanisms of bacterial toxin action, intracellular bacterial pathogens

7. Vaccines as tools for protection from infection, modulation of host response and immunotherapy

8. Antibiotics as anti-infectious therapeutics and resistance to antibiotics

9. Intestinal microflora, commensals - the human microbiome

10. Most studied bacterial pathogens - an introductory overview:

Corynebacterium diphteriae, Streptococcus pyogenes a Staphylococcus aureus

11. Most studied bacterial pathogens:

Clostridium botulinum/perfringens/tetani, B. cereus, Helicobacter pylori

12. Most studied bacterial pathogens:

Ricketsia, Fracisella, Coxiella, Yersinia, Salmonella, Shigella,

13. Most studied bacterial pathogens:

Campylobacter jejuni, Vibrio cholerae, Escherichia coli - EPEC, ETEC, UPEC, NMEC

14. Most studied bacterial pathogens:

Bordetella, Mycobacterium, Neisseria, Listeria, Borelia, Mycoplasma, Chlamydia

Registration requirements -
Last update: Šebo Peter prof. Ing. CSc. (30.01.2018)

Biochemistry, Microbiology and Molecular biology

Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 1 28
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Defense of an individual project 100

 
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