SubjectsSubjects(version: 880)
Course, academic year 2020/2021
Advanced bioanalytical methods - M402023
Title: Pokročilé bioanalytické metody
Guaranteed by: Department of Analytical Chemistry (402)
Actual: from 2020
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0 Ex [hours/week]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Setnička Vladimír prof. Ing. Ph.D.
Svoboda Petr Ing. Ph.D.
Šantrůček Jiří Ing. Ph.D.
Annotation -
Last update: Pátková Vlasta (05.01.2018)
Bioanalytical methods are applied to determination of analytes using specific interactions between molecules. Modern bioanalytical techniques provide high specificity of analysis (one of reagents is biopolymer and that is why we talk about biospecificity), but also high sensitivity and low detection limit. Part of lectures will cover enzymatic methods, proteomics, metabolomics, electromigration methods, advanced genetic methods, immunochemical and other biochemical and microbiological methods including modern trends as clinical biodiagnostics and modern individualized therapy approach.
Aim of the course -
Last update: Pátková Vlasta (05.01.2018)

Students will be able to:

Choose suitable special analytical method with appropriate detection limit.

Apply the chosen method to samples of different origin and their characterization.

Orientate themselves in specialized literature concerning biomolecules issue and special approach to their study.

Obtain wide knowledge of most modern bioanalytical methods spectra including possibility, suitability and limits of their application.

Literature -
Last update: Pátková Vlasta (05.01.2018)

R: Králová B., Rauch P., Fukal L., Bioanalytická chemie, Vydavatelství VŠCHT Praha, 2007, ISBN 80-7080-449-1

R: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Praha 2005, ISBN 80-7080-586-2

R: Ruml T., Rumlová M., Pačes V., Genové inženýrství, Praha 2002, ISBN 80-7080-499-8

R: Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418

A: Fukal L. - (Editor), Bioafinitní metody, Vysoká škola chemicko-technologická v Praze, Vydavatelství VŠCHT, 2005, ISBN: 80-7080-584-6.

A: Gault v., McClenaghan N., Understanding Bioanalytical Chemistry: Principles and Applications, Wiley, 2009, ISBN: 978-0-470-02906-0.

A: Racek J., Klinická biochemie, Galén 2006, ISBN 80-7262-324-9

Learning resources -
Last update: Pátková Vlasta (05.01.2018)

Additional list of suggested bibliography and electronic references will be provided within individual lectures.

Syllabus -
Last update: Svoboda Petr Ing. Ph.D. (16.04.2020)

1. Introduction to bioanalytical methods.

2. Analysis and quantification of biomoleculs.

3. Heavy metals and their influence on health.

4. Proteomics.

5. Metabolomics.

6. Mass spectrometry in bioanalysis.

7. Chromatography of biomolecules II.

8. Genetic methods II.

9. Recombinant peptides and proteins.

10. Cell culture.

11. Principles and application of electrophoresis.

12. Immunochemical techniques II.

13. Clinical biodiagnostics, modern individualized therapy.

14. Future direction of bioanalytical methods.

Registration requirements -
Last update: Pátková Vlasta (05.01.2018)

Analytical chemistry I

Biochemistry I

Bioanalytical methods N402016 (N320006)

Course completion requirements -
Last update: Svoboda Petr Ing. Ph.D. (28.05.2020)

Written examination

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 2 56
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 10
Examination test 90