SubjectsSubjects(version: 877)
Course, academic year 2020/2021
  
Foodborne Pathogens: Laboratory - N320012
Title: Laboratoř mikrobiologického zkoumání potravin
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2006
Semester: summer
Points: summer s.:2
E-Credits: summer s.:2
Examination process: summer s.:
Hours per week, examination: summer s.:0/3 MC [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Pazlarová Jarmila doc. RNDr. CSc.
Z//Is interchangeable with: M320028
Annotation -
Last update: TAJ320 (11.12.2013)
The subject targets to the identification and quantification of chosen food-born pathogens according to valid EU ISO norms. The subjekt is also focused on molecular biology and immunochemical methods of identification and to the detail characterization of studied microorganisms.
Aim of the course -
Last update: TAJ320 (11.12.2013)

Students will be able to identify and characterize important foodborn pathogens according to the respctive ISO standards.

Literature -
Last update: UST320 (25.09.2013)

Commission Regulation (EC) No 1441/2007 of 5 December 2007 amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs

R: Valid ČSN ISO norms

Learning resources -
Last update: TAJ320 (25.09.2013)

none

Syllabus -
Last update: UST320 (23.08.2013)

1. Safety rules in microbiological laboratory, preparation of sterile medium and instruments.

2. Preparation of defined, selective and complex media.

3. Sampling, homogenization of food samples, dilutions, inoculation.

4. Processing of food samples (ready to eat food- RTF).

5. Processing of food produce for heat treatment.

6. Processing of beverages samples.

7. Determation of total count of aerobic and facultatively anaerobic mezophilic bacteria, determination of coliform bacteria and yeasts and molds.

8. Performance of standard cultivation determination following ISO norms of: Bacillus cereus, Escherichia coli, Staphylococcus aureus.

9. Performance of standard cultivation determination following ISO norms of:Salmonella sp., Listeria monocytogenes.

10. Quantitative evaluation of all performed analysis and their assesssment.

11. Lateral flow immunochemical test for confirmation of Listeria monocytogenes and Salmonella sp.

12. Membrane filtration of water, cultivation and evaluation of samples.

13. Rapid identification methods for the identification of food-born pathogens.

14. Principles of qualitative and quantitative procedures of polymerase chain reaction - PCR for the detection of food-born pathogens. Disposal od all used cultures and media.

Registration requirements -
Last update: UST320 (19.09.2013)

Exam from Microbiology

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
2 / 2 56 / 56
Coursework assessment
Form Significance
Regular attendance 90
Report from individual projects 10

 
VŠCHT Praha