Advanced Biochemistry: Laboratory - N320036
Title: Laboratoř speciální biochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2020
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:0/5, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Level:  
Is provided by: M320012
Guarantor: Lipov Jan doc. Ing. Ph.D.
Novotná Zuzana doc. Dr. Ing.
Is interchangeable with: M320012
Examination dates   Schedule   
Annotation -
Tha aim of the subject is to expand the basic knowledge of the biochemical course and to adopt new techniques of modern biochemistry and to amend the spectrum of techniques of biochemistry and molecular biology used in research laboratories.
Last update: Lipov Jan (08.08.2013)
Aim of the course -

Students will be able to:

isolate the proteins from various sources

characterize the enzyme activity

determine the degree of cooperativity of the enzyme during its denaturation

identify the protein by mass spectrometry

isolate plasmid DNA and characterize it by restriction cleavage

analyze and process the results to protocols

Last update: TAJ320 (26.09.2013)
Literature -

R: Káš J., Kodíček M., Valentová O.: Laboratorní techniky biochemie. 1. vyd. Vysoká škola chemicko-technologická v Praze, Praha 2006. ISBN 80-7080-586-2

Last update: Lipov Jan (08.08.2013)
Learning resources -

Kodíček Milan: Biochemické pojmy : výkladový slovník (http://vydavatelstvi.vscht.cz/knihy/uid_es-002/)

Last update: Lipov Jan (08.08.2013)
Syllabus -

1. Good laboratory practice, safety

2. Theoretical introduction to the laboratory tasks

3. Chromatography of biomacromolecules, evaluation of purification steps

4. Electrophoretic analysis of erythrocyte membrane proteins

5. Basic characterization of purified preparates of proteins

6. Enzyme kinetics (allosteric enzymes)

7. Modern methods of protein identification

8. Physical & chemical properties of proteins

9. Immunotechniques

10.Basic techniques of molecular biology

11.Isolation of subcellular particles

12.Study of basic metabolic events

13.Methods of determination of catalytical activity of enzymes

14.Evaluation, test and free discussion

Last update: TAJ320 (26.09.2013)
Registration requirements -

Biochemistry I

Laboratory of biochemistry

Last update: TAJ320 (26.09.2013)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Report from individual projects 50
Examination test 50