SubjectsSubjects(version: 954)
Course, academic year 2023/2024
Chemical Food Safety: Laboratory - N323014
Title: Laboratoř chemické bezpečnosti potravin
Guaranteed by: Department of Food Analysis and Nutrition (323)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2021
Semester: summer
Points: summer s.:4
E-Credits: summer s.:4
Examination process: summer s.:
Hours per week, examination: summer s.:0/6, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: cancelled
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Is provided by: M323003
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Pulkrabová Jana prof. Ing. Ph.D.
Class: Základní laboratoře
Is interchangeable with: M323003
Examination dates   Schedule   
Annotation -
The students are after introductory instructing divided into working groups in which they execute particular tasks focused on the analysis of a selected group of compounds using various analytical methods.
Last update: HOLADOVK (16.07.2013)
Aim of the course -

Students will be able to:

  • apply modern analytical methods.
  • to evaluate and interpret the results in accordance to the requirements of practice.
Last update: TAJ323 (16.08.2013)
Literature -

R: All the materials and instructions needed are handed over at the beginning of the course.

Last update: HOLADOVK (16.07.2013)
Learning resources -

All the materials and instructions needed to fulfill lab exercises are handed over at the beginning of the course.

Last update: HOLADOVK (16.07.2013)
Syllabus -

1. The analysis of food additives and contaminants - introduction.

2. Training focused on gas chromatograph GC) equipped with electron-capture detector (ECD), nitrogen-phosphorus detector (NPD), flame-ionization detector (FID) and mass spectrometric detector (MSD).

3. Training focused on liquid chromatograph (LC) equipped with spectrophotometric detector (UV/VID) and evaporative light scattering detector (ELSD).

4. Training focused on liquid chromatograph (LC) equipped with mass-spectrometric detector (MSD).

5. Training focused on gel permeation chromatography (GPC, hydrophobic gel Bio Beads S-X3), adsorption chromatography (LSC, Florisil), solid phase extraction (SPE), solid phase microextraction (SPME) and dispersive solid phase extraction (QuEChERS).

6. Determination of free sulphur dioxide in wine using a titrimetric method.

7. Determination of antioxidants in vegetable oil using a GC/FID method after GPC clean-up.

8. a) Determination of sorbic and benzoic acid using HPLC/UV after SPE clean-up.

b) Determination of functional organic nanoparticles with the content of preserving agents and vitamins using HPLC/UV method and size-exclusion chromatography and using UPLC/ELSD method.

9. Determination of polar pesticides using LC/MS. a) Analysis of raw extracts. b) Analysis of the extracts cleaned up by QuEChERS. c) Determination of pesticides based quarternary amonium salts.

10. Determination of endogenous and exogenous contaminats using LC/MS. a) Analysis of fusarium mycotoxins deoxynivalenol (DON) and zearalenone in cereals. b) Analysis of free and masked acrylamide in food. c) Analysis of perfluorinated compounds.

11. Determination of polychlorinated biphenyls in pork using Stanovení GC/ECD method after LSC clean-up.

12. Determination of pesticides using SPME/GC/MS method. a) Analysis of organophosphate pesticides in rice. b) Analysis of ethylenbis(dithiocarbamates) in baby food.

13. Determination of organophosphate pesticides in rice using GC/NPD method after liquid-liquid extraction.

14. Evaluation of the lab course.

Last update: TAJ323 (16.08.2013)
Registration requirements -

Special Food Analysis; Chemical Food safety

Last update: TAJ323 (23.08.2013)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 3 84
Práce na individuálním projektu 1 28
4 / 4 112 / 112
Coursework assessment
Form Significance
Report from individual projects 100