SubjectsSubjects(version: 861)
Course, academic year 2019/2020
Chromatography and mass spectrometry: Laboratory - N323055
Title: Laboratoř chromatografie a hmotnostní spektrometrie
Guaranteed by: Department of Food Analysis and Nutrition (323)
Actual: from 2019
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:0/5 MC [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type:  
Guarantor: Pulkrabová Jana doc. Ing. Ph.D.
Stránská Milena doc. Ing. Ph.D.
Z//Is interchangeable with: M323022
Examination dates   Schedule   
Annotation -
Last update: Pulkrabová Jana doc. Ing. Ph.D. (03.07.2013)
Subject Chromatography and mass spectrometry: Laboratory is designed for students who have completed course Interpretation of chromatographic and mass-spectrometric data. The aim of the course is to practically introduce to students modern techniques for detection of compounds, data handling and interpretation, including statistical data processing (multivariate analysis). Laboratories are designed as single handle activities of students on their own projets.
Aim of the course -
Last update: TAJ323 (16.08.2013)

Students will be able to:

  • prepare and plan experimental work in the modern laboratory.
  • under the leadership to analyse organic compounds using the modern instruments.
  • process and interpret the measured data.
Literature -
Last update: Pulkrabová Jana doc. Ing. Ph.D. (03.07.2013)

R: Picó Y.: Food Toxicants Analysis, Elsevier, 2007, ISBN: 978-0-444-52843-8.

R: Otleş S.: Handbook of Food Analysis Instruments, CRC, 2008, ISBN: 9781420045666.

R: de Hoffmann E., Stroobant V.: Mass Spectrometry - Principles and Applications, Wiley, 2002 ISBN: 9780471485667.

R: Kaliszan R.: Structure and Retention in Chromatography: A Chemometric Approach (Chromatography: Principles & Practice Series), CRC, 1997 ISBN: 9789057020285.

Learning resources -
Last update: Pulkrabová Jana doc. Ing. Ph.D. (03.07.2013)

Syllabus -
Last update: TAJ323 (28.08.2013)

1. Introduction, organization of laboratories, laboratory safety, project assignment

2. - 3. Preparation of project (selection methods, literature search

4. - 11. Laboratory work - work on the project

12. - 14. Data processing, preparation of the final project report

Registration requirements -
Last update: Pulkrabová Jana doc. Ing. Ph.D. (03.07.2013)

Interpretation of chromatographic and spectrometric data

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 2 56
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,5 14
Práce na individuálním projektu 0,5 14
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 50
Defense of an individual project 50