SubjectsSubjects(version: 916)
Course, academic year 2022/2023
Analysis by Origin: Biological Samples - N402026
Title: Analýza z hlediska původu vzorku: biologické materiály
Guaranteed by: Department of Analytical Chemistry (402)
Faculty: Faculty of Chemical Engineering
Actual: from 2021
Semester: winter
Points: winter s.:2
E-Credits: winter s.:2
Examination process: winter s.:
Hours per week, examination: winter s.:1/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type:  
Guarantor: Sinica Alla Mgr. Ph.D.
Is interchangeable with: M402009
Examination dates   Schedule   
Annotation -
Last update: Sinica Alla Mgr. Ph.D. (23.09.2013)
This course is focused on understanding the principles of modern operating procedures and bioanalytical methods that are used in the analysis of complex biological samples for the determination of various analytes in biological matrix. The basis of this subject is to introduce the student with modern instrumental analytical methods for isolation, identification and determination of biologically important compounds.
Aim of the course -
Last update: VED402 (16.12.2013)

Students will expand and obtain new interdisciplinary knowledge in analytical and bioanalytical chemistry allowing them better orientation in the world of modern chemistry and to find applications in routine bioanalytical laboratories and in various fields of scientific and research activity, too.

Literature -
Last update: VED402 (01.10.2013)

R: Ahuja S., Jespersen N., Modern Instrumental Analysis, Elsevier, 2006, ISBN: 978-0-444- 52259-7

R: Manz A., Pamme N., Iossifidis D., Bioanalytical chemistry, Imperial College Press, 2004, ISBN 1-86044-3705

A: Mikkelsen S.R., Cortón E., Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA,2004, ISBN 0-471-54447-7

A: Suelter C. H., Methods of Biochemical Analysis: Bioanalytical Instrumentation, Volume 37, John Wiley & Sons, Inc., 2006, ISBN 9780471582601

Learning resources -
Last update: Sinica Alla Mgr. Ph.D. (23.09.2013)

Analytical and Bioanalytical Chemistry (

Mikkelsen S.R., Cortón E., Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA,2004 (

Syllabus -
Last update: Sinica Alla Mgr. Ph.D. (10.09.2012)

1. Basic methods of organic analysis. Specifics for biological materials

2. Preparation of biological samples for analysis.Importance and application of markers in analysis of biological materials

3. Methods of biological sample enrichment on monitored analyte

4. Separation procedures used for biological materials

5. Tracking methods, derivatisation procedures, radioactive and non-radioactive labeling

6. Determination of biologically important low molecular weight components

7. Detection and determination of the most significant carcinogens

8. Analysis of significant classes of drugs and their metabolites

9. Analysis of biopolymers and determination of their structure

10. Analysis of optical purity of samples, enantioselective separation and chiroptical methods

11. Methods of immunochemical analysis

12. Affinity chromatography and electro-chromatographic methods

13. Analytical methods in bioengineering

14. Analysis of combinatorial databases

Registration requirements -
Last update: Sinica Alla Mgr. Ph.D. (23.09.2013)

Analytická chemie I, II.

Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0,2 6
Obhajoba individuálního projektu 0,3 8
Účast na přednáškách 0,5 14
Práce na individuálním projektu 0,5 14
Příprava na zkoušku a její absolvování 0,5 14
2 / 2 56 / 56
Coursework assessment
Form Significance
Regular attendance 20
Defense of an individual project 30
Examination test 50