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This course is focused on understanding the principles of modern operating procedures and bioanalytical methods that are used in the analysis of complex biological samples for the determination of various analytes in biological matrix. The basis of this subject is to introduce the student with modern instrumental analytical methods for isolation, identification and determination of biologically important compounds.
Last update: Sinica Alla (23.09.2013)
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Students will expand and obtain new interdisciplinary knowledge in analytical and bioanalytical chemistry allowing them better orientation in the world of modern chemistry and to find applications in routine bioanalytical laboratories and in various fields of scientific and research activity, too. Last update: VED402 (16.12.2013)
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R: Ahuja S., Jespersen N., Modern Instrumental Analysis, Elsevier, 2006, ISBN: 978-0-444- 52259-7
R: Manz A., Pamme N., Iossifidis D., Bioanalytical chemistry, Imperial College Press, 2004, ISBN 1-86044-3705
A: Mikkelsen S.R., Cortón E., Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA,2004, ISBN 0-471-54447-7
A: Suelter C. H., Methods of Biochemical Analysis: Bioanalytical Instrumentation, Volume 37, John Wiley & Sons, Inc., 2006, ISBN 9780471582601 Last update: VED402 (01.10.2013)
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1. Basic methods of organic analysis. Specifics for biological materials 2. Preparation of biological samples for analysis.Importance and application of markers in analysis of biological materials 3. Methods of biological sample enrichment on monitored analyte 4. Separation procedures used for biological materials 5. Tracking methods, derivatisation procedures, radioactive and non-radioactive labeling 6. Determination of biologically important low molecular weight components 7. Detection and determination of the most significant carcinogens 8. Analysis of significant classes of drugs and their metabolites 9. Analysis of biopolymers and determination of their structure 10. Analysis of optical purity of samples, enantioselective separation and chiroptical methods 11. Methods of immunochemical analysis 12. Affinity chromatography and electro-chromatographic methods 13. Analytical methods in bioengineering 14. Analysis of combinatorial databases Last update: Sinica Alla (10.09.2012)
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Analytical and Bioanalytical Chemistry (http://www.springer.com/chemistry/analytical/journal/216) Mikkelsen S.R., Cortón E., Bioanalytical Chemistry, John Wiley & Sons, Inc., Hoboken, NJ, USA,2004 (http://onlinelibrary.wiley.com/book/10.1002/0471623628) Last update: Sinica Alla (23.09.2013)
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Analytická chemie I, II. Last update: Sinica Alla (23.09.2013)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Konzultace s vyučujícími | 0.2 | 6 | ||
Obhajoba individuálního projektu | 0.3 | 8 | ||
Účast na přednáškách | 0.5 | 14 | ||
Práce na individuálním projektu | 0.5 | 14 | ||
Příprava na zkoušku a její absolvování | 0.5 | 14 | ||
2 / 2 | 56 / 56 |
Coursework assessment | |
Form | Significance |
Regular attendance | 20 |
Defense of an individual project | 30 |
Examination test | 50 |