Subjects

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Genetic Engineering - AM320017

Title:	Genetic Engineering
Guaranteed by:	Department of Biochemistry and Microbiology (320)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2023
Semester:	winter
Points:	winter s.:3
E-Credits:	winter s.:3
Examination process:	winter s.:
Hours per week, examination:	winter s.:2/0, Ex [HT]
Capacity:	35 / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	English
Teaching methods:	full-time
Teaching methods:	full-time
Level:
Note:	course can be enrolled in outside the study plan enabled for web enrollment

Guarantor:	Ruml Tomáš prof. Ing. CSc.
Classification:	Biology > Genetics
Interchangeability :	M320017, N320051
Is interchangeable with:	M320017

Examination dates

This subject contains the following additional online materials

E-learning course

Annotation -

The subject is focused on understanding of principles of manipulation with nucleic acids and their analysis. It covers information about various methods of introducing genes into diverse cell types, determination of gene expression and analysis. The goal is to deliver information about genetic engineering techniques in order to facilitate the choce of the optimal method for a particular application. The lectures cover basic methods of isolation, analysis and modification of nucleic acids and special applications as gene modification for detection or affinity purification of gene product, study of interaction of proteins and nucleic acids, or gene therapies.

Last update: Kubová Petra (25.04.2018)

Aim of the course -

Students will be able to:

Isolate DNA and perform basic manipulations with recombinant DNA. Prepare vectors for genetic modification of various cell types.

Modify and label DNA, apply DNA labeled probes for identification of specific sequences.

Modify genes by directed mutagenesis by directed mutagenesis, extension by sequences for detection and purification by affinity chromatography.

Analyze production of heterologous proteins and isolate them from various cell types.

Analyze protein interactions. Construct gene libraries.

Last update: Kubová Petra (25.04.2018)

Course completion requirements -

Passing written test with a score exceeding 50% and passing an oral exam

Last update: Kubová Petra (25.04.2018)

Literature -

Green M. R., Sambrook J., Molecular Cloning: A Laboratory Manual (Fourth Edition); Cold Spring Harbor Laboratory Press 2012

Last update: Ruml Tomáš (17.05.2018)

Syllabus -

1. Recapitulation of genetic principles.

2. Bacterial plasmid vectors.

3. Basic operations with DNA.

4. Labeling of nucleic acids and application of probes.

5. Analysis of DNA sequences.

6. Principles of work with RNA.

7. Polymerase chain reaction and its applications.

8. Expression in microbial cells, markers, fusion proteins, reporter genes.

9. Tissue cultures and their use for gene expression.

10. Principle of construction of transgenic organisms.

11. Mutations, directed mutagenesis.

12. Genome mapping, restriction analysis.

13. Product detection - metabolic labeling, electrophoretic and immunochemical methods.

14. Methods for study in protein and nucleic acids interactions.

Last update: Kubová Petra (25.04.2018)

Learning resources -

http://biomikro.vscht.cz/vyuka/?Predmet=gieng

Last update: Kubová Petra (25.04.2018)

Registration requirements -

Biochemistry

Molecular genetics or Molecular genetics and DNA analysis

Last update: Kubová Petra (25.04.2018)