SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Atomic Spectrometry - AM402001
Title: Atomic Spectrometry
Guaranteed by: Department of Analytical Chemistry (402)
Actual: from 2019
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Extent per academic year: 2 [hours]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Mestek Oto prof. Ing. CSc.
Interchangeability : M402001, N402005, S402005
Is interchangeable with: M402001
Annotation -
Last update: Kubová Petra Ing. (22.01.2018)
In lectures, students learn about modern methods of trace element analysis based on the principles of atomic spectroscopy. Introductory part of the course is devoted to theoretical principles, the structure of the atom, energy transitions in the electron shell of atoms and basic structural elements used in atomic spectroscopy. The first part of the course comprises atomic absorption spectroscopy. Students learn the used radiation sources, methods of atomization interference correction methods and some special approaches to the analysis (generation of volatile compounds, electrolytic deposition of analyte). Another series of lectures is devoted to emission spectral analysis, students will learn various methods of excitation spectra, such as spark, arc, glow discharge, plasma and laser. The following part of the lectures if concerned with inductively coupled plasma mass spectrometry, the students will learn the basics of instrumentation, methods of correction of spectral and non-spectral interferences and special techniques, such as elemental speciation analysis, single-particle analysis and isotope dilution. Attention is also paid to the atomic fluorescence spectroscopy and the X-ray spectroscopy. In the lectures on applications, students get acquainted with work in the trace lab, the methods of preparation of various types of samples (water, biological materials, ores and rocks) and the possibilities of using atomic spectroscopy techniques for in situ analysis.
Aim of the course -
Last update: Kubová Petra Ing. (22.01.2018)

Students completing the course will acquire theoretical knowledge of the methods of elemental analysis using optical spectroscopy (absorption, emission and fluorescence), X-ray spectrometry and mass spectrometry. They will be familiar with the various methods of sample preparation and problems of cleanness in trace laboratory.

Literature -
Last update: Mestek Oto prof. Ing. CSc. (20.02.2018)

R: Cullen M.: Atomic Spectroscopy in Elemental Analysis, Blackwell, ISBN 1-84127-333-3

R: José A.C. Broekaert: Analytical Atomic Spectrometry with Flames and Plasmas, Wiley, ISBN 978-3-527-31282-5

Skripta (in Czech): Černohorský T., Jandera P.: Atomová spektroskopie, Univerzita Pardubice 1997. ISBN 80-7194-114-X

R: Ebdon L., Evans E.H., Fisher A.S., Hill S.J.: An Introduction to Analytical Atomic Spectrometry. Willey 1998, ISBN 978-0-471-97418-5

Learning resources -
Last update: Kubová Petra Ing. (22.01.2018)

http://web.vscht.cz/~koplikr/Atomov%c3%a1%20%20spektrometrie.pdf

Requirements to the exam -
Last update: Kubová Petra Ing. (22.01.2018)

An oral examination. Student can use only two reparative terms.

Syllabus -
Last update: Kubová Petra Ing. (22.01.2018)

1. Structure of atoms.

2. Interaction of electrons and electromagnetic radiation, width and intensity of spectral lines.

3. Fundamentals of optics, construction of monochromators and polychromators, construction of radiation detectors.

4. Atomic absorption spectroscopy, basic instrumentation - radiation sources, atomizers.

5. Atomic absorption spectroscopy: interference and background correction, signal optimization and processing.

6. Atomic absorption spectroscopy: special techniques - hydride generation, mercury determination, electrodeposition.

7. Atomic fluorescence spectroscopy.

8. Flame emission spectroscopy, spectroscopy with electric arc and spark excitation, rotating electrode spectroscopy, spectroscopy with glow discharge.

9. Inductively coupled and DC plasma optical emission spectroscopy, LIBS

10. Inductively coupled plasma mass spectroscopy: basic instrumentation - ion optics, ion filters and detectors.

11. Inductively coupled plasma mass spectroscopy: interference and methods of their elimination; special techniques - laser ablation, analysis of nanoparticles, connection with electrothermal evaporation.

12. Hyphenated techniques in speciation analysis of trace elements.

13. X-ray fluorescence spectrometry.

14. Preparation of samples for elemental trace analysis, principles of work in a trace laboratory.

Registration requirements -
Last update: Kubová Petra Ing. (22.01.2018)

Basic knowledge of analytical chemistry

Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0,5 14
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,5 14
Příprava na zkoušku a její absolvování 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Oral examination 100

 
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