SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Recent trends in isolation and separation methods - AP323008
Title: Recent trends in isolation and separation methods
Guaranteed by: Department of Food Analysis and Nutrition (323)
Actual: from 2019
Semester: winter
Points: winter s.:0
E-Credits: winter s.:0
Examination process: winter s.:
Hours per week, examination: winter s.:3/0 other [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Level:  
For type: doctoral
Guarantor: Poustka Jan prof. Dr. Ing.
Pulkrabová Jana doc. Ing. Ph.D.
Interchangeability : P323008
Annotation -
Last update: Pátková Vlasta (15.11.2018)
The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors, mass spectrometry.
Aim of the course -
Last update: Pátková Vlasta (15.11.2018)

Students will be able to understand:

1. Principles, technical configurations and application potential of sample preparation methods

2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors

3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation

Literature -
Last update: Pátková Vlasta (15.11.2018)

R: Grob, R.L. (Editor) : Modern Practice of Gas Chromatography, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-59700-7, 1995

R: Dean, J.R. : Extraction Methods for Environmental Analysis, J.Wiley&Sons, Ltd., Chichester, Anglie, ISBN 0-471-98287-3, 1998

R: J. Pawliszyn: Solid phase microextraction: Theory and Practice. Wiley-VCH, Inc. . NY, USA, ISBN 0-471-19034-9, 1997

R: Willoughby, R., Sheehan, E., Mitrovich, S.: A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems, Global View Publishing, Pittsburg, Pennsylvania, USA, 1998.

R: Snyder,L.R., Kirkland, J.J., Glajch, J.L.: Practical HPLC Method Development, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-00703-X, 1997

A: Rood, D.: A Practical Guide to the Care Maintenance and Troubleshooting of Capillary Gas Chromatographic Systems, Hüthig GmbH, Heidelberg, Německo, ISBN 3-7785-2319-8, 1995

A: Scientific papers

Learning resources -
Last update: Pátková Vlasta (15.11.2018)

Course outlines: http://web.vscht.cz/poustkaj

Teaching methods -
Last update: Pátková Vlasta (15.11.2018)

Lectures, consultations, self-study.

Requirements to the exam -
Last update: Pátková Vlasta (15.11.2018)

No requirements.

Syllabus -
Last update: Pátková Vlasta (15.11.2018)

1. Overview of analytical methods and techniques in analytical procedures

2. Isolation methods in gas phase - purge & trap (PT), head space (HS)

3. Supercritical fluid extraction (SFE)

4. Liquid extraction – techniques: liquid-liquid extraction (LLE), liquid-solid extraction (LSE)

5. Liquid extraction at elevated temperature and pressure (ASE-PLE, MASE)

6. Solid phase extraction (SPE); solid phase micro-extraction (SPME)

7. Adsorption chromatography (LSC); gel permeation chromatography (GPC)

8. Dispersive solid phase extraction (MSPD, QuEChERS)

9. Gas chromatography – parameters; separation process, injection techniques; columns, detectors; GCxGC

10. Liquid chromatography – parameters; separation process, columns, detectors

11. Mass spectrometry – ionisation; mass analyzers, spectra interpretation

Entry requirements -
Last update: Pátková Vlasta (15.11.2018)

Basic knowledge of the fields: general chemistry, inorganic chemistry, organic chemistry, analytical chemistry.

Registration requirements -
Last update: Pátková Vlasta (15.11.2018)

No requirements.

Course completion requirements -
Last update: Pátková Vlasta (15.11.2018)

Exam - written test.

 
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