SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Recent trends in isolation and separation methods - P323008
Title: Moderní trendy v izolačních a separačních metodách
Guaranteed by: Department of Food Analysis and Nutrition (323)
Actual: from 2019
Semester: winter
Points: winter s.:0
E-Credits: winter s.:0
Examination process: winter s.:
Hours per week, examination: winter s.:3/0 other [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: doctoral
Guarantor: Poustka Jan prof. Dr. Ing.
Pulkrabová Jana doc. Ing. Ph.D.
Is interchangeable with: AP323008
Annotation -
Last update: Poustka Jan prof. Dr. Ing. (22.05.2018)
The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors, mass spectrometry.
Aim of the course -
Last update: Poustka Jan prof. Dr. Ing. (22.05.2018)

Students will be able to understand:

1. Principles, technical configurations and application potential of sample preparation methods

2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors

3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation

Literature -
Last update: Poustka Jan prof. Dr. Ing. (14.06.2018)

R: Grob, R.L. (Editor) : Modern Practice of Gas Chromatography, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-59700-7, 1995

R: Dean, J.R. : Extraction Methods for Environmental Analysis, J.Wiley&Sons, Ltd., Chichester, Anglie, ISBN 0-471-98287-3, 1998

R: J. Pawliszyn: Solid phase microextraction: Theory and Practice. Wiley-VCH, Inc. . NY, USA, ISBN 0-471-19034-9, 1997

R: Willoughby, R., Sheehan, E., Mitrovich, S.: A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems, Global View Publishing, Pittsburg, Pennsylvania, USA, 1998.

R: Snyder,L.R., Kirkland, J.J., Glajch, J.L.: Practical HPLC Method Development, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-00703-X, 1997

A: Rood, D.: A Practical Guide to the Care Maintenance and Troubleshooting of Capillary Gas Chromatographic Systems, Hüthig GmbH, Heidelberg, Německo, ISBN 3-7785-2319-8, 1995

A: Scientific papers

Learning resources -
Last update: Poustka Jan prof. Dr. Ing. (14.06.2018)

Course outlines: http://web.vscht.cz/poustkaj

Teaching methods -
Last update: Poustka Jan prof. Dr. Ing. (14.06.2018)

Lectures, consultations, self-study.

Requirements to the exam -
Last update: Vlčková Martina Ing. (19.07.2018)

No requirements.

Syllabus -
Last update: Poustka Jan prof. Dr. Ing. (22.05.2018)

1. Overview of analytical methods and techniques in analytical procedures

2. Isolation methods in gas phase - purge & trap (PT), head space (HS)

3. Supercritical fluid extraction (SFE)

4. Liquid extraction – techniques: liquid-liquid extraction (LLE), liquid-solid extraction (LSE)

5. Liquid extraction at elevated temperature and pressure (ASE-PLE, MASE)

6. Solid phase extraction (SPE); solid phase micro-extraction (SPME)

7. Adsorption chromatography (LSC); gel permeation chromatography (GPC)

8. Dispersive solid phase extraction (MSPD, QuEChERS)

9. Gas chromatography – parameters; separation process, injection techniques; columns, detectors; GCxGC

10. Liquid chromatography – parameters; separation process, columns, detectors

11. Mass spectrometry – ionisation; mass analyzers, spectra interpretation

Entry requirements -
Last update: Poustka Jan prof. Dr. Ing. (14.06.2018)

Basic knowledge of the fields: general chemistry, inorganic chemistry, organic chemistry, analytical chemistry.

Registration requirements -
Last update: Poustka Jan prof. Dr. Ing. (14.06.2018)

No requirements.

Course completion requirements -
Last update: Poustka Jan prof. Dr. Ing. (22.05.2018)

Exam - written test.

Coursework assessment
Form Significance
Examination test 100

 
VŠCHT Praha