SubjectsSubjects(version: 853)
Course, academic year 2019/2020
  
Molecular genetics and DNA analysis - B320015
Title: Molekulová genetika a analýza DNA
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2019
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Bachelor's
Guarantor: Ruml Tomáš prof. Ing. CSc.
Interchangeability : AB320015, N320081
This subject contains the following additional online materials
Annotation -
Last update: Ruml Tomáš prof. Ing. CSc. (26.01.2018)
The subject is focused on understanding heredity principles especially mechanisms and regulation of transfer of genetic information and gene expression in prokaryotic and eukaryotic organisms. Special emphasis will be put on the main regulatory processes mediated by proteins and RNA regulatory molecules. Other focus is on understanding the variability of genetic information due to mutations and recombination. The subject offers information on the most important methods of DNA analysis as DNA identification and sequencing. The students will learn basic basic principles of pharmacogenomics and oncogenesis.
Aim of the course -
Last update: Ruml Tomáš prof. Ing. CSc. (26.01.2018)

Students will know:

Basic principles of molecular genetics and the relationship of their structure and function.

Principles of regulation of transfer of genetic information and its variability due to mutations and recombination

Mechanisms involved in mutation repair.

Basic processes of gene expression as regulation of transcription, in prokaryotes and eukaryotes and posttranscriptional modifications of eukaryotic mRNA, regulation of stability of mRNA and translation, role of small RNAs.

Basic principles of DNA analysis and basic applications of polymerase chain reaction

Basic principles of pharmacogenomics and oncogenesis

Literature -
Last update: Kubová Petra Ing. (27.03.2019)

R: Lodish, H.; et al. Molecular cell biology, 8th ed.;W.H.Freeman & Co Ltd, 2016.

Learning resources -
Last update: Ruml Tomáš prof. Ing. CSc. (26.01.2018)

Berk A., Lodish H., Kaiser Ch. A.: (2016) Molecular Cell Biology, 8th Edition, Palgrave Macmillan

Syllabus -
Last update: Ruml Tomáš prof. Ing. CSc. (30.01.2018)

1. Historic overview and basic genetic laws and terms.

2. Structure and function of nucleic acids, genome organization

3. Replication, DNA polymerases, replisome complex.

4. Mutations and repair mechanisms.

5. Recombination, gene segregation.

6. Transposition, transduction, regulation of yeast mating type.

7. Regulation of prokaryotic transcription.

8. Eukaryotic transcription, posttranscriptional modifications of RNA.

9. Regulatory roles of RNA.

10. Translation.

11. Comparison of prokaryotic and eukaryotic translation, post-translational modification.

12. DNA analysis for identification of persons.

13. Fundamentals of pharmacogenomics.

14. Genetic principles of cancer.

Entry requirements -
Last update: Kubová Petra Ing. (15.01.2018)

To enroll in the course it is required enrollment in courses N110004 A N320004

Registration requirements -
Last update: Kubová Petra Ing. (15.01.2018)

Biochemistry I

Course completion requirements -
Last update: Hochel Igor doc. Ing. CSc. (05.03.2018)

completed written and oral exams

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,5 15
Příprava na zkoušku a její absolvování 1,3 35
3 / 3 78 / 84
Coursework assessment
Form Significance
Examination test 40
Oral examination 60

 
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