SubjectsSubjects(version: 853)
Course, academic year 2019/2020
  
Molecular genetics and DNA analysis - N320081
Title: Molekulová genetika a analýza DNA
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2013
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Guarantor: Ruml Tomáš prof. Ing. CSc.
Annotation -
Last update: Ruml Tomáš prof. Ing. CSc. (13.09.2019)
The subject is focused on understanding heredity principles especially mechanisms and regulation of transfer of genetic information and gene expression in prokaryotic and eukaryotic organisms. Special emphasis will be put on the main regulatory processes mediated by proteins and RNA regulatory molecules. Other focus is on understanding the variability of genetic information due to mutations and recombination. The subject offers information on the most important methods of DNA analysis as DNA identification, sequencing and analysis of selected sequences by using polymerase chain reaction.
Aim of the course -
Last update: TAJ320 (19.09.2013)

Students will know:

Basic principles of molecular genetics and the relationship of their structure and function.

Principles of regulation of transfer of genetic information and its variability due to mutations and recombination

Mechanisms involved in mutation repair.

Basic processes of gene expression as regulation of transcription, in prokaryotes and eukaryotes and posttranscriptional modifications of eukaryotic mRNA, regulation of stability of mRNA and translation, role of small RNAs.

Basic principles of work with DNA, methods of DNA analysis and basic applications of polymerase chain reaction

Literature -
Last update: Ruml Tomáš prof. Ing. CSc. (13.09.2019)

Michael J Simmons, Peter D. Snustad: Genetika, Nakladatel: Masarykova univerzita Brno 2017

Lodish, H.; et al. Molecular cell biology, 8th ed.;W.H.Freeman & Co Ltd, 2016.

Albrets B; et al. Molecular biology of the cell, 5th ed. Garland Science, 2008.

Clark D.P. Molecular biology, Elsevier Inc., 2005.

Hitler J.M. Forensic DNA Typing, 2nd ed. Elsevier 2005.

Learning resources -
Last update: TAJ320 (26.09.2013)

none

Syllabus
Last update: Ruml Tomáš prof. Ing. CSc. (13.09.2019)

1. Historical overview, basic genetic concepts and laws

2. Structure and function of nucleic acids, genome organization

3. Replication I, general features, prokaryotes

4. Replication II, eukaryotes, replisome complex

5. Recombination I, gene segregation in meiosis

6. Recombination II, transposition

7. Mutation and repair mechanisms

8. Methods of DNA sequence analysis, genome mapping

9. Regulation of transcription

10. Posttranscriptional modification of RNA

11. Translation I, initiation, elongation and termination, differences between prokaryotes and eukaryotes

12. Translation II, posttranslational modifications

13. Basic principles of pharmacogenomics

14. Basics of oncogenesis

Entry requirements -
Last update: TAJ320 (19.09.2013)

To enroll in the course it is required enrollment in courses N110004 A N320004

Registration requirements -
Last update: TAJ320 (26.09.2013)

Biochemistry I

Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0 1
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,8 21
Příprava na zkoušku a její absolvování 1,1 31
3 / 3 81 / 84
Coursework assessment
Form Significance
Examination test 40
Oral examination 60

 
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