SubjectsSubjects(version: 845)
Course, academic year 2018/2019
  
Molecular genetics and DNA analysis - N320081
Title in English: Molekulová genetika a analýza DNA
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2013
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Guarantor: Ruml Tomáš prof. Ing. CSc.
This subject contains the following additional online materials
Annotation -
Last update: TAJ320 (11.12.2013)
The subject is focused on understanding heredity principles especially mechanisms and regulation of transfer of genetic information and gene expression in prokaryotic and eukaryotic organisms. Special emphasis will be put on the main regulatory processes mediated by proteins and RNA regulatory molecules. Other focus is on understanding the variability of genetic information due to mutations and recombination. The subject offers information on the most important methods of DNA analysis as DNA and RNA isolation, their labeling, sequencing and analysis of selected sequences by using polymerase chain reaction and comparison of data with available databases.
Aim of the course -
Last update: TAJ320 (19.09.2013)

Students will know:

Basic principles of molecular genetics and the relationship of their structure and function.

Principles of regulation of transfer of genetic information and its variability due to mutations and recombination

Mechanisms involved in mutation repair.

Basic processes of gene expression as regulation of transcription, in prokaryotes and eukaryotes and posttranscriptional modifications of eukaryotic mRNA, regulation of stability of mRNA and translation, role of small RNAs.

Basic principles of work with DNA, methods of DNA analysis and basic applications of polymerase chain reaction

Literature -
Last update: TAJ320 (26.09.2013)

R: Lodish, H.; et al. Molecular cell biology, 6th ed.;W.H.Freeman & Co Ltd, 2008.

R: Albrets B; et al. Molecular biology of the cell, 5th ed. Garland Science, 2008.

R: Clark D.P. Molecular biology, Elsevier Inc., 2005.

R: Hitler J.M. Forensic DNA Typing, 2nd ed. Elsevier 2005.

Learning resources -
Last update: TAJ320 (26.09.2013)

none

Syllabus
Last update: TAJ320 (19.09.2013)

1. Historic overview and basic genetic laws and terms.

2. Structure and function of nucleic acids, genome organization

3. Replication, DNA polymerases, replisome complex.

4. Mutations and repair mechanisms.

5. Recombination, mitotic and meiotic gene segregation, transposition.

6. Regulation of transcription.

7. Posttranscriptional modifications of RNA.

8. Regulatory role of RNA.

9. Translation, posttranslational modifications.

10. Principles of work with DNA and RNA.

11. Methods of analysis of DNA sequence, genome mapping.

12. Labeling of nucleic acids, application of probes.

13. Polymerase chain reaction and its applications.

14. DNA databases.

Entry requirements -
Last update: TAJ320 (19.09.2013)

To enroll in the course it is required enrollment in courses N110004 A N320004

Registration requirements -
Last update: TAJ320 (26.09.2013)

Biochemistry I

Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0 1
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,8 21
Příprava na zkoušku a její absolvování 1,1 31
3 / 3 81 / 84
Coursework assessment
Form Significance
Zkouškový test 40
Ústní zkouška 60

 
VŠCHT Praha