SubjectsSubjects(version: 847)
Course, academic year 2018/2019
  
Biochemistry: Seminar I - N320003
Title in English: Seminář z biochemie I
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2017
Semester: winter
Points: winter s.:1
E-Credits: winter s.:1
Examination process: winter s.:
Hours per week, examination: winter s.:0/1 MC [hours/week]
Capacity: unknown / unlimited (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Additional information: http://předmět je vyučován pouze v zimním semestru
Guarantor: Lipov Jan Ing. Ph.D.
Benešová Eva Ing. Ph.D.
Interchangeability : Z320003
Annotation -
Last update: Lipov Jan Ing. Ph.D. (08.08.2013)
Biochemistry: Seminar I is mainly focused on practical knowledge necessary for successful mastery of basic laboratory techniques. It familiarizes students with various separation techniques and immunochemical methods and allows the understanding of their real use in practice using specific examples. In the second half the seminar is focused on natural biocatalysts - enzymes, on their classification, kinetics and inhibition, again using examples from laboratory work.
Aim of the course -
Last update: TAJ320 (26.09.2013)

Students will be able to:

draw formulas and evaluate charge properties of coded aminoacids

draw the titration curve of peptides

understand basic chromatography techniques (gel permeation, ion exchange, affinity)

understand basics of immunochemical methods

divide enzymes to the classes

understand basics of enzyme kinetics and inhibition

Literature -
Last update: Lipov Jan Ing. Ph.D. (08.08.2013)

R: Káš J., Kodíček M., Valentová O.: Laboratorní techniky biochemie. 1. vyd. Vysoká škola chemicko-technologická v Praze, Praha 2006. ISBN 80-7080-586-2

A: Nelson D. L., Cox M. M., Principles of Biochemistry, 4. vydání, W.H.Freeman&Co., New York, 2005, 978-0716762652

Learning resources -
Last update: Lipov Jan Ing. Ph.D. (08.08.2013)

Kodíček Milan: Biochemické pojmy : výkladový slovník (http://vydavatelstvi.vscht.cz/knihy/uid_es-002/)

Syllabus -
Last update: TAJ320 (26.09.2013)

1. Introduction

2. Aminoacids (structure, classification, peptide formation)

3. Aminoacids (charge properties, isoelectric point)

4. Aminoacids (titration curves, aminoacids as buffers)

5. Separation techniques Ia (gel permeation and ion exchange chromatography)

6. Separation techniques Ib (other chromatochraphy techniques)

7. Separation techniques II - electrophoresis

8. Immunochemical methods

9. Determination of protein concentration

10. Determination of protein primary structure

11. Enzymes (basic properties, classification to classes, nomenclature)

12. Enzyme kinetics (examples)

13. Enzyme inhibition (examples)

14. Final summary and practice

Registration requirements -
Last update: Benešová Eva Ing. Ph.D. (26.08.2013)

none

Teaching methods
Activity Credits Hours
Příprava na zkoušku a její absolvování 0,5 14
Účast na seminářích 0,5 14
1 / 1 28 / 28
Coursework assessment
Form Significance
Continuous assessment of study performance and course -credit tests 100

 
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