SubjectsSubjects(version: 876)
Course, academic year 2020/2021
  
Immunochemistry: Laboratory - N320026
Title: Laboratoř imunochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2009
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:0/4 MC [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Guarantor: Hochel Igor doc. Ing. CSc.
Z//Is interchangeable with: M320004
Annotation -
Last update: Hochel Igor doc. Ing. CSc. (11.07.2013)
The course is focused on i) acquiring of knowledge and skills in the discipline, ii) detailed description of antigen, hapten and all significant isotypes of antibodies, iii) utilization of antibodies for isolation and preparation of pure preparations, iv) utilization of antibodies in food and clinical testing and environmental monitoring, v) mastering the procedures of various immunochemical assays.
Aim of the course -
Last update: Hochel Igor doc. Ing. CSc. (09.07.2013)

Students will be able to:

Design the working procedures and to apply immunochmical methods in food and clinical control and in environmental testing.

Literature -
Last update: Hochel Igor doc. Ing. CSc. (09.07.2013)

R: Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418

Learning resources -
Last update: Hochel Igor doc. Ing. CSc. (11.07.2013)

Other study aids:

protocols: http://biomikro.vscht.cz/vyuka/?Predmet=fm

Syllabus -
Last update: Hochel Igor doc. Ing. CSc. (09.07.2013)

1. Immunoprecipitation in liquid phase

2. Double diffusion according to Ouchterlony

3. Radial immunodiffusion according to podle Mancini

4. Electrosyneresis

5. Immunoelectrodiffusion according to Laurell

6. Indirect competitive ELISA

7. Non-competitive ELISA

8. GLISA Rapid test

9. SDS-PAGE of proteins, blotting and immunochemical detection of analytes

10. Immunoagglutination

Registration requirements -
Last update: Hochel Igor doc. Ing. CSc. (09.07.2013)

Biochemistry, Microbiology

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 2 56
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,5 14
Příprava na zkoušku a její absolvování 0,5 14
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 50
Report from individual projects 25
Examination test 25

 
VŠCHT Praha