SubjectsSubjects(version: 954)
Course, academic year 2019/2020
Fundamentals of Bioengineering - AB319002
Title: Fundamentals of Bioengineering
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2020
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: not taught
Language: English
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Halecký Martin doc. Ing. Ph.D.
Interchangeability : B319002, N319002
Is interchangeable with: B319002, S319008
Examination dates   Schedule   
This subject contains the following additional online materials
Annotation -
The course focuses on understanding the whole process of industrial bioprocesses e.g. cell cultivation, upstream and downstream processes, process interactions and engineering aspects of the process. The subject is based on expansion, synthesis and application of knowledge gained in basic subjects such as microbiology, biochemistry, physical chemistry and chemical engineering to bioengineering aspects of industrial bioprocesses. The subject brings new or significantly expanding existing knowledge in the field of mass balance bioprocesses, types of bioreactors and methods of management and control of bioprocesses.
Last update: Halecký Martin (16.09.2023)
Aim of the course -

Students will be able to:

Apply knowledge of basic subjects such as microbiology, biochemistry and chemical engineering to bioengineering area.

Propose a suitable type of cultivation and bioreactor for the desired product.

Design and implement a mass balance and basic process control.

Last update: Halecký Martin (16.09.2023)
Literature -

R: Moo-Young M., (2019) Comprehensive Biotechnology. Elsevier, ISBN: 9780444640475

R: Najafpour, G. D. (2015). Biochemical Engineering and Biotechnology. Elsevier, ISBN: 9780444633774

R: Katoh S., Horiuchi J., & Yoshida F. (2015). Biochemical Engineering: A Textbook for Engineers, Chemists and Biologists. Wiley, ISBN: 9783527338047

Last update: Halecký Martin (16.09.2023)
Learning resources -

Last update: Halecký Martin (16.09.2023)
Syllabus -

1. Biotechnology and bioengineering, biotechnological process structure.

2. Technological aspects of use of intact cells as a producers (plant vs. animal vs. microorganisms). Types of products. Application of enzymes.

3. Upstream processes (storage of microorganisms, preparation of inoculum, preparation and sterilization of liquid media, sterilization of reactor, SIP, filtration and sterilization of gases).

4. Parameters for the description of microorganism cultivation. Yield and productivity of process. Overview of cultivation types.

5. Batch cultivation (characteristics, mass balance, use).

6. Fed-batch cultivation (characteristics, types of feeding, mass balance, use).

7. Continuous cultivation (characteristics, process control, mass balance, compared to batch cultivation, use). Choosing the cultivation type on the basis of the desired product.

8. Aeration and oxygen transfer (implementation of aeration, oxygen transfer mechanism, KLa).

9. Mixing (mixing types in biotechnological plant, specifics of mixing in bioreactors).

10. Bioreactors (types, design, use).

11. Measured and controlled variables in cell cultivation. Fundamentals of bioprocess control (controllers, control loop, variables in controlling).

12. Downstream processes (conventional separation technologies, disintegration of cells, membrane separation technologies).

13. Downstream processes (extraction, precipitation, distillation, drying, stripping, CIP). Production line of biotechnological process.

14. Biotechnologies and bioreactors for treatment of wastes from biotechnological processes.

Last update: Halecký Martin (16.09.2023)
Registration requirements -

Physical chemistry I

Biochemistry I

Last update: Halecký Martin (16.09.2023)
Course completion requirements -

Credit test; test and oral exam.

Last update: Halecký Martin (16.09.2023)