SubjectsSubjects(version: 954)
Course, academic year 2019/2020
Fundamentals of Bioengineering - N319002
Title: Základy bioinženýrství
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2013 to 2020
Semester: both
Points: 4
E-Credits: 4
Examination process:
Hours per week, examination: 2/1, C+Ex [HT]
Capacity: winter:48 / 48 (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Additional information: http://Předmět je vyučován pouze v zimním semestru!
Note: you can enroll for the course in winter and in summer semester
Guarantor: Halecký Martin doc. Ing. Ph.D.
Is interchangeable with: B319002, AB319002
Examination dates   Schedule   
Annotation -
The course focuses on understanding the whole process of industrial bioprocesses e.g. cell cultivation, upstream and downstream processes, process interactions and engineering aspects of the process. The subject is based on expansion, synthesis and application of knowledge gained in basic subjects such as microbiology, biochemistry, physical chemistry and chemical engineering to bioengineering aspects of industrial bioprocesses. The subject brings new or significantly expanding existing knowledge in the field of mass balance bioprocesses, types of bioreactors and methods of management and control of bioprocesses.
Last update: Halecký Martin (13.08.2013)
Aim of the course -

Students will be able to:

Apply knowledge of basic subjects such as microbiology, biochemistry and chemical engineering to bioengineering area.

Propose a suitable type of cultivation and bioreactor for the desired product.

Design and implement a mass balance and basic process control.

Last update: Halecký Martin (13.08.2013)
Literature -

R: Kaštánek F., Bioinženýrství, Academia, Praha, 2008, ISBN: 80-200-0768-7

R: Najafpour G. D., Biochemical Engineering and Biotechnology, Elsevier B.V., Amsterdam, 2007, ISBN: 978-0-444-52845-2

R: Rehm H.-J., Reed G., Biotechnology, Volume 3 - Bioprocessing WILEY-VCH, 1993, ISBN: 9783527283101

R: Rehm H.-J., Reed G., Biotechnology, Volume 4 - Measuring, Modelling and Control, Second Edition, WILEY-VCH, 1991, ISBN: 9783527283101

Last update: Halecký Martin (14.11.2014)
Learning resources -

Last update: Halecký Martin (13.08.2013)
Syllabus -

1. Biotechnology and bioengineering, biotechnological process structure.

2. Technological aspects of use of intact cells as a producers (plant vs. animal vs. microorganisms). Types of products. Application of enzymes.

3. Upstream processes (storage of microorganisms, preparation of inoculum, preparation and sterilization of liquid media, sterilization of reactor, SIP, filtration and sterilization of gases).

4. Parameters for the description of microorganism cultivation. Yield and productivity of process. Overview of cultivation types.

5. Batch cultivation (characteristics, mass balance, use).

6. Fed-batch cultivation (characteristics, types of feeding, mass balance, use).

7. Continuous cultivation (characteristics, process control, mass balance, compared to batch cultivation, use). Choosing the cultivation type on the basis of the desired product.

8. Aeration and oxygen transfer (implementation of aeration, oxygen transfer mechanism, KLa).

9. Mixing (mixing types in biotechnological plant, specifics of mixing in bioreactors).

10. Bioreactors (types, design, use).

11. Measured and controlled variables in cell cultivation. Fundamentals of bioprocess control (controllers, control loop, variables in controlling).

12. Downstream processes (conventional separation technologies, disintegration of cells, membrane separation technologies).

13. Downstream processes (extraction, precipitation, distillation, drying, stripping, CIP). Production line of biotechnological process.

14. Biotechnologies and bioreactors for treatment of wastes from biotechnological processes.

Last update: Halecký Martin (29.09.2014)
Registration requirements -

Physical chemistry I

Biochemistry I

Last update: Halecký Martin (13.08.2013)
Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.4 10
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0.9 25
Příprava na zkoušku a její absolvování 1.2 34
Účast na seminářích 0.5 14
4 / 4 111 / 112
Coursework assessment
Form Significance
Regular attendance 30
Continuous assessment of study performance and course -credit tests 20
Oral examination 50