SubjectsSubjects(version: 955)
Course, academic year 2019/2020
Fundamentals of Bioengineering - B319002
Title: Základy bioinženýrství
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2020
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: not taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Halecký Martin doc. Ing. Ph.D.
Interchangeability : AB319002, N319002
Is interchangeable with: AB319002
Examination dates   Schedule   
This subject contains the following additional online materials
Annotation -
The course focuses on understanding the whole process of industrial bioprocesses e.g. cell cultivation, upstream and downstream processes, process interactions and engineering aspects of the process. The subject is based on expansion, synthesis and application of knowledge gained in basic subjects such as microbiology, biochemistry, physical chemistry and chemical engineering to bioengineering aspects of industrial bioprocesses. The subject brings new or significantly expanding existing knowledge in the field of mass balance bioprocesses, types of bioreactors and methods of management and control of bioprocesses.
Last update: Halecký Martin (16.09.2023)
Aim of the course -

Students will be able to:

Apply knowledge of basic subjects such as microbiology, biochemistry and chemical engineering to bioengineering area.

Propose a suitable type of cultivation and bioreactor for the desired product.

Design and implement a mass balance and basic process control.

Last update: Halecký Martin (16.09.2023)
Literature -

R: Moo-Young M., (2019) Comprehensive Biotechnology. Elsevier, ISBN: 9780444640475

R: Najafpour, G. D. (2015). Biochemical Engineering and Biotechnology. Elsevier, ISBN: 9780444633774

R: Katoh S., Horiuchi J., & Yoshida F. (2015). Biochemical Engineering: A Textbook for Engineers, Chemists and Biologists. Wiley, ISBN: 9783527338047

Last update: Halecký Martin (01.07.2024)
Learning resources -

Last update: Halecký Martin (16.09.2023)
Syllabus -

1. Structure of the biotechnological process in terms of unit operations. Engineering aspects of the use of intact cells and enzymes as producers.

2. Equipment and unit upstream operations – sterilization of liquid medium and bioreactor, SIP station, filtration and sterilization of gases. Parameters to describe the cultivation.

3. Batch cultivation – characteristics, mass balance, applications.

4. Fed-batch cultivation – characteristics, feed strategies, mass balance, applications.

5. Continuous cultivation – characteristics, process control methods, applications.

6. Continuous cultivation – mass balance, comparison with batch and fed-batch cultivation, process variations.

7. Aeration and oxygen transfer – implementation of aeration, mechanism of oxygen transfer mass balance.

8. Mixing – types of mixing in biotechnology plants, specifics of bioreactor mixing.

9. Bioreactors – classification, design, application; pneumatically and mechanically stirred aerated bioreactors.

10. Bioreactors – photobioreactors, membrane bioreactors, tissue culture reactors, reactors for biotechnology in waste management and environment.

11. Measured and controlled variables in cell cultivation. Basics of bioprocess control – control circuit, types of controllers, variables in control.

12. Equipment and unit downstream operations – cleaning, CIP station, conventional separation technology, cell disintegration.

13. Equipment and unit downstream operations – membrane separation technologies, extraction, precipitation, distillation, drying, stripping.

14. Methods of optimization and scale-up of bioprocesses.

Last update: Halecký Martin (11.09.2021)
Registration requirements -

Physical chemistry I

Biochemistry I

Last update: Halecký Martin (16.09.2023)
Course completion requirements -

Credit test; test and oral exam.

Last update: Halecký Martin (31.01.2018)
Teaching methods
Activity Credits Hours
Účast na přednáškách 1.5 42
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0.9 26
Příprava na zkoušku a její absolvování 1.1 30
Účast na seminářích 0.5 14
4 / 4 112 / 112
Coursework assessment
Form Significance
Regular attendance 20
Examination test 30
Continuous assessment of study performance and course -credit tests 20
Oral examination 30