SubjectsSubjects(version: 882)
Course, academic year 2020/2021
  
Biology: Laboratory - AB320004
Title: Biology: Laboratory
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2019
Semester: summer
Points: summer s.:1
E-Credits: summer s.:1
Examination process: summer s.:
Hours per week, examination: summer s.:0/2 MC [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Level:  
For type: Bachelor's
Additional information: http://předmět je vyučován pouze v letním semestru
Guarantor: Burketová Lenka doc. Ing. CSc.
Kotrba Pavel prof. Ing. Ph.D.
Interchangeability : B320004, N320015, S320015
Is interchangeable with: B320004
Annotation -
Last update: Kubová Petra Ing. (06.05.2019)
The course provides training in the methods of light and fluorescence microscopy, good laboratory practice and rules governing organization of a biological experiment. The course objectives further include: inspection of structure of plant and animal cells and tissues, microscopy and cytochemistry of cellular processes, and analysis of chromosomal basis of heredity.
Aim of the course -
Last update: Kubová Petra Ing. (06.05.2019)

Students will be able to:

Describe principles the light and fluorescence microscopy lie in and will be knowledgeable in principal parameters of a light microscope.

Independently prepare microscope specimens, inspect them through the light and fluorescence microscopy, organize an experiment and properly record and interpret the observations.

Better understand organization of the cell, plant and animal tissues, mitosis and chromosomal basis of heredity, which were all in theory the subjects of preceding Biology course.

Literature -
Last update: Kubová Petra Ing. (06.05.2019)

Z:Kotrba P., Babůrek I., Knejzlík Z., Návody ke cvičením z biologie, VŠCHT Praha, 2006, 8070806230

D:Benda V., Babůrek I., Kotrba P., Základy biologie, VŠCHT Praha, 2005, 8070805870

Learning resources -
Last update: Kubová Petra Ing. (06.05.2019)

Anonymous, Boundless Biology Open Textbook, boundless.com, free acess at https://www.boundless.com/biology/

Teaching methods -
Last update: Kubová Petra Ing. (06.05.2019)

Attendance at laboratory courses

Syllabus -
Last update: Kubová Petra Ing. (06.05.2019)

1. Introduction to Light Microscopy, Fluorescence Microscopy and Other Microscopy Techniques

2. Specimen Preparation for Light Microscopy

3. Microscopy of the Cell, Morphology and Inclusion Bodies of the Cell

4. Microscopy of Organelles of the Cell with Light Microscope

5. Microscopy of Organelles of the Cell using Fluorescence Microscopy

6. Cytochemical Staining of Macromolecules and Detection of Enzyme Activity

7. Osmosis in Cells

8. Cell Types and Intercellular Space in Plant Tissues

9. Anatomy of Stems, Roots and Leaves and Vascular Tissue in Plants

10. Histology � Epithelial and Connective Tissues, Blood Smear

11. Histology � Muscle and Nervous Tissues

12. Cytogenetics � Animal Chromosomes and Karyotype

13. Mitosis in Plant Meristem and Cells of Animal Tissue Culture

14. Exercises in Plant, Animal and Human Genetics

Registration requirements -
Last update: Kubová Petra Ing. (06.05.2019)

none

Course completion requirements -
Last update: Kubová Petra Ing. (06.05.2019)

Passing the exam consisting of a written and oral examination.

 
VŠCHT Praha