SubjectsSubjects(version: 901)
Course, academic year 2021/2022
  
Biology: Laboratory - B320004
Title: Laboratoř biologie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2021 to 2021
Semester: summer
Points: summer s.:1
E-Credits: summer s.:1
Examination process: summer s.:
Hours per week, examination: summer s.:0/2 MC [hours/week]
Capacity: unknown / 156 (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Bachelor's
Additional information: http://předmět je vyučován pouze v letním semestru
Guarantor: Angelini Jindřiška PharmDr. Ph.D.
Interchangeability : AB320004, N320015
Is interchangeable with: AB320004
This subject contains the following additional online materials
Annotation -
Last update: Angelini Jindřiška PharmDr. Ph.D. (23.02.2022)
The course provides training in the methods of light and fluorescence microscopy, good laboratory practice and rules governing the organization of a biological experiment. The course objectives further include: inspection of structure of plant and animal cells and tissues, microscopy and cytochemistry of cellular processes in practical and theoretical tasks. This course not only develops students' spatial imagination, but also teaches them to abstract and record important scientific information of the reality observed in the microscope.
Aim of the course -
Last update: Kotrba Pavel prof. Ing. Ph.D. (17.01.2018)

Students will be able to:

Describe principles the light and fluorescence microscopy lie in and will be knowledgeable in principal parameters of a light microscope.

Independently prepare microscope specimens, inspect them through the light and fluorescence microscopy, organize an experiment and properly record and interpret the observations.

Better understand organization of the cell, plant and animal tissues, mitosis and chromosomal basis of heredity, which were all in theory the subjects of preceding Biology course.

Literature -
Last update: Kotrba Pavel prof. Ing. Ph.D. (17.01.2018)

Z:Kotrba P., Babůrek I., Knejzlík Z., Návody ke cvičením z biologie, VŠCHT Praha, 2006, 8070806230

D:Benda V., Babůrek I., Kotrba P., Základy biologie, VŠCHT Praha, 2005, 8070805870

Learning resources -
Last update: Kotrba Pavel prof. Ing. Ph.D. (17.01.2018)

Anonymous, Boundless Biology Open Textbook, boundless.com, free acess at https://www.boundless.com/biology/

Teaching methods -
Last update: Kotrba Pavel prof. Ing. Ph.D. (17.01.2018)

Attendance at laboratory courses

Syllabus -
Last update: Angelini Jindřiška PharmDr. Ph.D. (23.02.2022)

1 a) Introduction to light microscopy, fluorescence microscopy, and other microscopic techniques

1 b) Preparation of samples for light microscopy

1 c) Microscopy of plant epidermal structures, starch and pollen grains

2 a) Cytology and imaging methods of subcellular structures

2 b) Microscopy of plant organelles and calcium oxalate crystals

2 c) Protoplasmic streaming and osmotic phenomena in the cell

3 a) Plant anatomy and organology

3 b) Plant tissues and their imaging

3 c) Microscopic structure of the stem, root, and leaf

4 a) Cytochemistry and the cell cycle

4 b) Microscopy of the stages of mitosis in the meristem

4 c) Visualization of plant polymers and enzyme functionality

5 a) Basics of human cell histology

5 b) Microscopic comparison of healthy and pathologically altered tissues

Registration requirements -
Last update: Kotrba Pavel prof. Ing. Ph.D. (17.01.2018)

none

Course completion requirements -
Last update: Angelini Jindřiška PharmDr. Ph.D. (04.02.2022)

Passing the exam consisting of a written and oral examination.

Formally and substantively correct protocol on samples

observed in the practical course.

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
1 / 1 28 / 28
 
VŠCHT Praha