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The course provides training in the methods of light and fluorescence microscopy, good laboratory practice and rules governing organization of a biological experiment. The course objectives further include: inspection of structure of plant and animal cells and tissues, microscopy and cytochemistry of cellular processes, and analysis of chromosomal basis of heredity.
Last update: Kotrba Pavel (28.06.2013)
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Students will be able to: Describe principles the light and fluorescence microscopy lie in and will be knowledgeable in principal parameters of a light microscope. Independently prepare microscope specimens, inspect them through the light and fluorescence microscopy, organize an experiment and properly record and interpret the observations. Better understand organization of the cell, plant and animal tissues, mitosis and chromosomal basis of heredity, which were all in theory subjects of preceding Biology I course. Last update: Kotrba Pavel (09.07.2013)
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Z:Kotrba P., Babůrek I., Knejzlík Z., Návody ke cvičením z biologie, VŠCHT Praha, 2006, 8070806230 D:Benda V., Babůrek I., Kotrba P., Základy biologie, VŠCHT Praha, 2005, 8070805870 Last update: Kotrba Pavel (09.11.2012)
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1. Introduction to Light Microscopy, Fluorescence Microscopy and Other Microscopy Techniques 2. Specimen Preparation for Light Microscopy 3. Microscopy of the Cell, Morphology and Inclusion Bodies of the Cell 4. Microscopy of Organelles of the Cell with Light Microscope 5. Microscopy of Organelles of the Cell using Fluorescence Microscopy 6. Cytochemical Staining of Macromolecules and Detection of Enzyme Activity 7. Osmosis in Cells 8. Cell Types and Intercellular Space in Plant Tissues 9. Anatomy of Stems, Roots and Leaves and Vascular Tissue in Plants 10. Histology � Epithelial and Connective Tissues, Blood Smear 11. Histology � Muscle and Nervous Tissues 12. Cytogenetics � Animal Chromosomes and Karyotype 13. Mitosis in Plant Meristem and Cells of Animal Tissue Culture 14. Exercises in Plant, Animal and Human Genetics Last update: Kotrba Pavel (09.11.2012)
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Anonymous, Boundless Biology Open Textbook, boundless.com, free acess at https://www.boundless.com/biology/ Last update: Kotrba Pavel (09.07.2013)
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none Last update: Kotrba Pavel (09.07.2013)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
1 / 1 | 28 / 28 |
Coursework assessment | |
Form | Significance |
Report from individual projects | 30 |
Continuous assessment of study performance and course -credit tests | 70 |